A simple method for the extraction of mutagens from airborne particles |
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| Authors: | G. Krishna J. Nath T. Ong W-Z. Whong |
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| Affiliation: | (1) Division of Plant and Soil Sciences, College of Agriculture and Forestry, West Virginia University, 26506 Morgantown, WV, U.S.A.;(2) Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, 26505 Morgantown, WV, U.S.A. |
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| Abstract: | Organic materials were extracted with acetone from filters of airborne particles by soaking, shaking, soxhletion, and sonication. These extracts were tested with and without S9 for mutagenicity using Ames assay and arabinose-resistant assay of Salmonella typhimurium. Among the extraction methods, soaking extract had the highest mutagenic activity followed by sonication, shaking, and soxhletion in both the assays. With the samples studied, it was concluded that soaking with acetone for 1/2 hr is the simplest and an efficient procedure for the extraction of mutagens from airborne particles.Results of cooperative investigation of NIOSH and West Virginia Agricultural and Forstry Experimental Station. Published with the approval of the Director of the West Virginia Agricultural and Forestry Experiment Station as Scientific Paper No. 1822. |
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