| 铜绿假单胞菌ZGKD2的重金属耐性机制研究 |
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| 引用本文： | 张玉秀,王姣,柴团耀,张倩,刘金光,李霞,白志强,苏增健.铜绿假单胞菌ZGKD2的重金属耐性机制研究[J].环境科学,2012,33(10):3613-3619. |
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| 作者姓名： | 张玉秀 王姣 柴团耀 张倩 刘金光 李霞 白志强 苏增健 |
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| 作者单位： | 1. 中国矿业大学（北京）化学与环境工程学院,北京,100083
2. 中国科学院研究生院生命科学院,北京,100049 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08009-130B);国家高技术研究发展计划(863)项目(2009AA06Z320,2006AA10Z407);中央高校基本科研业务费专项(2010YH05);大学生创新性实验计划项目(110309z) |
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| 摘 要： | 从煤矸石中分离的耐Cd2+铜绿假单胞菌(Pseudomonas aeruginosa)ZGKD2具有多种重金属耐性,研究其铁载体产生特征和重金属耐性机制对于开发土壤重金属污染修复技术具有重要意义.在牛肉膏蛋白胨培养基中分别添加200～3 000μmol.L-1的Cd2+、Cu2+、Zn2+、Ni2+、Pb2+或Mn2+等重金属,ZGKD2菌株的最大生长量随着重金属浓度的增加而降低,不同浓度重金属对ZGKD2菌株产碱能力无明显影响.ZGKD2菌株生长进入稳定期时,用0、200、600、1 000μmol.L-1的Cd2+、Cu2+、Zn2+、Ni2+或0、1 000、2 000、3 000μmol.L-1的Pb2+、Mn2+处理2 h,SOD和CAT活性随重金属浓度增加而增加.ZGKD2菌株在MSA培养基中能产生铁载体,200～1 000μmol.L-1的重金属Cd2+或Zn2+可显著诱导铁载体的产生,Ni2+和Mn2+的作用较小,Cu2+则抑制;ZGKD2菌株的产铁载体能力与重金属耐性能力相关.这些结果表明ZGKD2菌株的产碱、产铁载体特性以及抗氧化酶活性提高可能是其耐重金属的主要机制.
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| 关 键 词： | 铜绿假单胞菌 重金属 抗氧化酶 铁载体 耐性 |
| 收稿时间： | 2011/12/22 0:00:00 |
| 修稿时间： | 2/6/2012 12:00:00 AM |
Mechanism of Heavy-Metal Tolerance in Pseudomonas aeruginosa ZGKD2 |
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| ZHANG Yu-xiu,WANG Jiao,CHAI Tuan-yao,ZHANG Qian,LIU Jin-guang,LI Xi,BAI Zhi-qiang and SU Zeng-jian.Mechanism of Heavy-Metal Tolerance in Pseudomonas aeruginosa ZGKD2[J].Chinese Journal of Environmental Science,2012,33(10):3613-3619. |
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| Authors: | ZHANG Yu-xiu WANG Jiao CHAI Tuan-yao ZHANG Qian LIU Jin-guang LI Xi BAI Zhi-qiang and SU Zeng-jian |
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| Institution: | School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China;School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China;College of Life Science, Graduate University of Chinese Academy of Sciences,Beijing 100049,China;School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China;School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China;School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China;School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China;School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijng 100083,China |
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| Abstract: | |
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| Keywords: | Pseudomonas aeruginosa heavy metal antioxidant enzyme siderophore tolerance |
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