二苯并噻吩脱硫微生物菌种的筛选与活性

从被石油污染的土壤中筛选出1株能对二苯并噻吩(DBT)进行高效脱硫的微生物菌种,初步鉴定为红串红球菌USTB-03(RhodococcuserythropolisUSTB-03).该菌种可以按特异性脱硫途径(简称4S途径)将DBT转化为2-羟基联苯(2HBP)和亚硫酸作为最终脱硫产物.在葡萄糖、甘油和乙醇分别作为微生物生长的唯一碳源时,葡萄糖是支持该菌生长和提高其脱硫比活性的较好碳源,使培养出的微生物对DBT的脱硫比活性达到了68.63mmol2HBP/(kg·h).该菌株还可以对4,6-二甲基二苯并噻吩(4,6-DMDBT)进行脱硫.

Screen and activity of dibenzothiophene by desulfurization microbe strain

A microbe strain which could desulfurize high-effectively the dibenzothiophene (DBT) was screened from the petroleum polluted site and was identified preliminarily as Rhodococcus erythropolis USTB-03. This strain could convert DBT to 2-hydroxybiphenyl (2HBP) and sulfurous acid as the final desulfurized products through specific desulfurization pathway (4s pathway). When glucose, glycerol and ethanol were respectively the sole carbon source of the microbe growth, glucose was the better carbon source for supporting the growth of this bacterium and enhancing its specific desulfurization activity; thus the specific actively of cultivated USTB-03 on DBT reached 68.63mmol 2HBP/(kg?h). This strain could also desulfurize 4,6-dimethyldibenzothiophene (4,6-DMDBT).