| pH值对亚硝酸盐氧化菌动力学及功能基因的影响 |
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| 引用本文： | 张昕,吴长峰,于雪,吕慧,陈桐生,孙洪伟.pH值对亚硝酸盐氧化菌动力学及功能基因的影响[J].中国环境科学,2020,40(4):1537-1544. |
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| 作者姓名： | 张昕 吴长峰 于雪 吕慧 陈桐生 孙洪伟 |
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| 作者单位： | 1. 兰州交通大学环境与市政工程学院, 甘肃 兰州 730070;
2. 甘肃省污水处理行业技术中心, 甘肃 兰州 730070;
3. 兰州城市供水(集团)有限公司;
4. 中山大学, 广东 广州 510000;
5. 环境保护部华南环境科学研究所, 广东 广州 510655;
6. 烟台大学环境与材料工程学院, 山东 烟台 264005 |
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| 基金项目： | 国家自然科学基金资助项目（51668031）；深圳市科技计划项目（JSGG20170824101458515） |
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| 摘 要： | 为探究pH值对亚硝酸盐氧化菌（NOB）活性动力学影响，本试验采用序批式活性污泥（SBR）反应器，以富含NOB的活性污泥为对象，基于Monod模型考察不同pH值对NOB活性动力学的影响并进行统计学分析.结果表明，Monod方程可较好地反映不同pH值条件下基质底物浓度对NOB比亚硝态氮氧化速率（SNiOR）的影响，且pH=7.0时动力学参数Ks为（6.167mg/L），rmax为1.134g/（g·d）]，此时NOB活性最好.利用钟形经验模型进行非线性回归拟合，最大比降解速率（rmax）随pH值的增大呈钟形变化，本试验NOB的最佳pH值为（6.9±0.1），其中rmax维持在ropt一半以上的pH值范围（ω）为（3.26±0.4）.以亚硝酸盐氧化还原酶类基因（nxrA、nxrB）为引物，基于荧光定量PCR技术分析结果显示，在不同pH值条件下nxrA基因和nxrB基因拷贝数的变化趋势均与动力学参数（Ks、rmax）的规律一致，且nxrA和nxrB基因在系统的降解过程中起协同作用.
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| 关 键 词： | 亚硝酸盐氧化菌 pH值 动力学 钟形经验模型 荧光定量PCR |
| 收稿时间： | 2019-10-08 |
Effects of pH on the kinetics of NOB and functional genes |
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| ZHANG Xin,WU Chang-feng,YU Xue,Lü Hui,CHEN Tong-sheng,SUN Hong-wei.Effects of pH on the kinetics of NOB and functional genes[J].China Environmental Science,2020,40(4):1537-1544. |
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| Authors: | ZHANG Xin WU Chang-feng YU Xue Lü Hui CHEN Tong-sheng SUN Hong-wei |
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| Institution: | 1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Gansu Sewage Treatment Industry Technical Center, Lanzhou 730070, China;
3. Lanzhou Urban Water Supply(Group) co. LTD, Lanzhou 730070, China;
4. Sun Yat-sen University, Guangzhou 510000, China;
5. South China Institute of Environmental Science, Guangzhou 510655, China;
6. School of Environment and Material Engineering, Yantai University, Yantai 264005, China |
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| Abstract: | A sequencing batch reactor (SBR) was operated in this study to investigate the effect of pH on the kinetics of NOB activity.For revealing the details the activated sludge abundant with NOB was used as an object and several statistics analyses based on Monod model were also applied. The results showed that the Monod equation fitted the experimental data well, and the greatest NOB activity was achieved as Ks=6.167mg/L, rmax=1.13g/(g·d) under pH=7.0.The analyses of bell-shaped empirical model disclosed that rmax varied in a bell shape as pH value increased. The optimum pH was 6.9±0.1whilethe rmax maintained above a half of the greatest rmax(ropt) was 3.26±0.4. The real-time PCR analysis results showed that the variation trend of nxrA gene and nxrB gene is consistent with the rule of kinetic parameters, and both nxrA and nxrB genes worked in the degradation process of the system. |
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| Keywords: | NOB pH kinetic bell-shaped empirical model real-time PCR |
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