饮用水常规处理工艺中细菌群落的时空分布

为弄清饮用水常规处理工艺过程中细菌群落的时空分布和动态变化规律，以我国南方某市一常规处理工艺水厂为研究对象，采用Illumina HiSeq高通量测序技术对夏季和冬季原水、沉后水、滤后水、出厂水和滤砂生物膜等细菌群落进行解析.结果表明，出厂水pH值、浊度、COD_Mn、菌落总数等指标均满足《生活饮用水卫生标准》（GB5749-2006）的要求.夏季细菌多样性明显高于冬季，混凝沉淀和消毒是影响细菌群落多样性和组成的主要工艺单元.细菌群落组成呈现一定的季节性变化，水样中优势菌门主要包括变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、蓝细菌门（Cyanobacteria）等，在滤砂生物膜样品中，变形菌门（Proteobacteria）亦占绝对优势.在属水平上，检测到的条件致病菌属包括弧形菌属（Vibrio）、不动杆菌属（Acinetobacter）和分支杆菌属（Mycobacterium）.水温和溶解氧是影响细菌群落的主要水质参数.

Spatiotemporal distribution bacterial communities in the conventional treatment process of drinking water

In order to gain insight into the spatiotemporal distribution and dynamic variation of bacterial communities in the conventional treatment process of drinking water, bacterial communities, which were in raw water, settling basin effluent, sand filter effluent and finished water in a conventional treatment process plant in the south of China in summer and winter, were investigated using Illumina HiSeq high throughput sequencing technology. The results showed that pH, turbidity, COD_Mn and total plate count in finished water all met the standards for drinking water quality (GB5749-2006). Bacterial diversity was significantly higher in summer than in winter. In addition, the coagulation sedimentation and disinfection process units had a significant effect on the composition and diversity of bacterial community. Furthermore, the composition of bacterial community varied seasonally, and the dominant bacterial phyla in water samples mainly included Proteobacteria, Actinobacteria and Cyanobacteria, etc, and Proteobacteria predominanted in filter sand biofilm samples. A number of potential pathogens were detected at the genus level, including Vibrio, Acinetobacte and Mycobacterium. Water temperature and dissolved oxygen were the major water characteristics that influenced bacterial community.