籼稻单倍体绿芽无性系二倍化的研究

以籼稻单倍体绿芽无性系为材料，研究秋水仙碱等处理对水稻植株染色体二倍化的影响．结果显示：（1）以500mg／L秋水仙碱溶液振荡处理籼稻单倍体绿芽48h，其二倍化率达60％～75％，处理时间过长或处理浓度过高．虽植株染色体二倍率有所提高，但二倍体植株的得率下降；（2）秋水仙碱在愈伤组织阶段处理，其染色体二倍率远高于非愈伤组织化的绿芽二倍率，高达96％～100％，其实未经处理的绿芽愈伤组织再生植株也能获得较高的加倍率；（3）在培养基中添加多效唑（2．5mg／L）也能增加籼稻植株染色体二倍率，由原来的3．3％提高到6．6％．

STUDY ON DIPLOIDIZATION OF HAPLOID GREEN SHOOT CLONES IN INDICA RICE

Haploid green shoots from long term subculture were used to study the effects of colchicine on diploidization of haploid green shoot clones. The results indicated that: (1 )When the haploid green shoots were shooked with sterile colchicine solution at concentration of 500 mg/L for 48 hours, the frequency of diploidization was 60% ~ 75%. The longer the treatment time or the greater the concentration of colchicine solution was used, the gnater the frequency of diploidization was reduced; (2)The frequency of diploidization at callus stagecould be as high as 96% ~ 100%. However, the frequency of diploidization of green shoot callus could be 74%; (3 )The frequency of diploidization in Indica rice increased from 3.3% to 6.6% with MET(2.5 mg/L)solution.