| 萘胁迫对水稻基因组DNA甲基化模式及水平的影响 |
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| 引用本文： | 范建成,刘宝,王隽媛,盛连喜.萘胁迫对水稻基因组DNA甲基化模式及水平的影响[J].环境科学,2010,31(3):793-800. |
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| 作者姓名： | 范建成 刘宝 王隽媛 盛连喜 |
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| 作者单位： | 东北师范大学城市与环境科学学院;东北师范大学国家环境保护湿地生态与植被恢复重点实验室;东北师范大学生命科学学院; |
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| 基金项目： | 国家水体污染控制与治理科技重大专项(2008ZX07207-009-03);;吉林省科技发展计划项目(20065021) |
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| 摘 要： | 运用MSAP技术测定水稻(Oryza sativa L.)纯系品种‘日本腈’和‘松前’经萘染毒胁迫后,不同生长时期叶片基因组DNA甲基化变异的情况.结果表明,水稻经萘胁迫后存在基于DNA甲基化水平和模式改变的表观遗传变异;5-甲基胞嘧啶百分含量的变化无统一趋势或规律;全部检测到的1051个位点中,日本腈有16.56%发生了变异,相对于松前的12.08%具有显著差异,一定程度上说明抗萘胁迫能力与基因型有关:松前强于日本腈;不同基因型及不同生长时期DNA甲基化模式的变异存在明显差异,表现为以去甲基化为主(0.48%~10.41%),超甲基化较少(0.10%~1.92%),由此推测DNA去甲基化可能是植物抗萘胁迫机制的一部分.
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| 关 键 词： | 水稻 日本腈 松前 萘 DNA甲基化 甲基化敏感扩增多态性 |
| 收稿时间： | 2008/12/25 0:00:00 |
| 修稿时间： | 2009/9/11 0:00:00 |
Effect of Napthalene on the DNA Methylation Patterns and Level in Rice (Oryza sativa L.) |
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| FAN Jian-cheng,LIU Bao,WANG Jun-yuan and SHENG Lian-xi.Effect of Napthalene on the DNA Methylation Patterns and Level in Rice (Oryza sativa L.)[J].Chinese Journal of Environmental Science,2010,31(3):793-800. |
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| Authors: | FAN Jian-cheng LIU Bao WANG Jun-yuan and SHENG Lian-xi |
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| Institution: | 1.College of Urban and Environmental Sciences;Northeast Normal University;Changchun 130024;China;2.State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration;3.College of Life Sciences;China |
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| Abstract: | In this study,two elite rice inbred lines,Nipponbare and Mastumae were selected and irrigated with naphthalene solution with different concentrations.Epigenetic instabilities in the blade of the plants in tillering and heading stage were assessed by MSAP marks.The results showed that naphthalene exposure induced epigenetic variations in all the samples based on different levels and patterns of DNA methylation.The changes of the percentage of 5-methylcytosine have no regulation.In total 1051 sites tested,the... |
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