野猪和几种家猪亲缘关系的RAPD分析

对二花脸猪、红灯笼猪、姜曲海猪、香猪、约克夏猪及华东地区野猪共２４个个体进行了随机扩增多态ＤＮＡ（ＲＡＰＤ）分析，以探讨家猪与野猪间的亲缘关系．实验从４０个引物中选用重复性较好的１３个引物对所有个体进行了随机扩增，共获得１９２个ＲＡＰＤ标记．ＲＡＰＤ研究结果表明：（１）长江下游江苏地区家猪品种或类群内遗传变异相对较小，群体的遗传趋异程度处于较低水平；（２）二花脸猪、红灯笼猪及姜曲海猪与华东地区的野猪可能起源于一个共同的祖先；（３）中国家猪各地方品种可能由分布于各地区的亚洲野猪经人们长期的定向选育而形成

STUDY ON PHYLOGENETIC RELATIONSHIP BETWEEN CHINESE WILD BOAR AND DOMESTIC PIGS USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

RAPDs were used to study the genetic divergency and phylogenetic relationship of Chinese wild boar and 5 breeds or strains of domestic pigs, including Erhualian pig, Hongdenglong pig, Jiangquhai pig, Xiang pig and Yorkshire. 24 individuals were used in this study, and the phylogenetic tree was reconstructed using UPGMA method based on 192 RAPD makers which were amplified with 13 decamer primers. The results indicate that: (1) The domestic pigs exhibit relatively low level of genetic variation and poor genetic diversity in Jiangsu in the lower reaches of the Yangtze River; (2) Erhualian, Hongdenglong and Jiangquhai pigs have close phylogenetic relationship with the Chinese wild boar in eastern China, and they may have a common ancestor; (3) The domestic pigs in China are likely to have originated from wild boars distributing in different areas of China, and were impacted by the directive breeding of people.