酚降解菌株的分离、鉴定和在含酚废水生物处理中的应用

从石油化工厂的活性污泥和废水混合物中分离到10个降解酚的细菌菌株,经16S rRNA基因序列分析,5个菌株(PD1、PD2、PD6、PD7和PD39)被鉴定为假单胞菌(Pseudomonas sp.),4个菌株(PD4、PD5、PD8和PD9)为不动杆菌(Acinetobacter sp.),1个菌株(PD3)为丛毛单胞菌(Comamonas sp.).对假单胞菌(Pseudomonas sp.)PD39菌株的酚降解特性、最适生长条件、降解底物的范围、儿茶酚1,2-双加氧酶(C12O)和儿茶酚2,3-双加氧酶(C23O)活性、含酚废水的生物处理等,进行了详细研究.结果表明,PD39菌株的最适生长和降解条件是,培养基的起始pH为7.0,培养温度为30℃,酚浓度为800 mg/L,对酚的最大降解浓度为1 200 mg/L.酚浓度为637 mg/L的工业废水经72 h处理酚的去除率达到99.96%.PD39菌株有很好的应用潜力来作含酚废水活性污泥处理系统的强化菌株.

Isolation and Identification of Phenol-degrading Strains and the Application in Biotreatment of Phenol-Containing Wastewater

Ten phenol-degrading bacterial strains were isolated from mixture of activated sludge and wastewater of a petroleum chemical plant. The five isolates (PD1, PD2, PD6, PD7 and PD39) were identified as Pseudomonas sp.,the four (PD4, PD5, PD8 and PD9) as Acinetobacter sp.,and the one (PD3) as Comamonas sp.by 16S rDNA sequence. Biodegradation characteristics of phenol, optimal conditions for growth, substrate range, activities of catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, and biotreatment of phenol-containing wastewater of Pseudomonas sp.PD39 were investigated in detail. The results indicated that the optimal conditions for growth and degradation of strain PD39 are beginning pH of medium 7.0, growth temperature 30 degrees C, concentration of phenol 800 mg/L. PD39 was capable of metabolizing phenol at concentrations up to 1200 mg/L and removing 637 mg/L in industrial wastewater by 99.96% in 72 h. This strain possesses a good application potential as a bioaugmentation strain in the activated sludge system for treatment of phenol-containing wastewater.