Differential activity of non-fluorinated and fluorinated analogues of the European corn borer pheromone |
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Authors: | Joan Solé Albert Sans Magí Riba Gloria Rosell Esmeralda Rosa Lourdes Muñoz Maria Pilar Bosch Angel Guerrero |
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Institution: | (1) Centre UdL–IRTA, Universitat de Lleida, Rovira Roure 191, 25198 Lleida, Spain;(2) Department of Pharmacology and Therapeutic Chemistry (Associated with CSIC), Faculty of Pharmacy, University of Barcelona, Av.Diagonal s/n, 08028 Barcelona, Spain;(3) Department of Biological Organic Chemistry, IIQAB (CSIC), Jordi Girona Salgado 18–26, 08034 Barcelona, Spain |
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Abstract: | Summary. The differing antagonist activity of (Z)-13-hexadecen-2-one (Z11 – 14 :MK, 1) and its 1,1,1-trifluoro derivative (Z11 –14 :TFMK, 2), two closely related analogues of the European corn borer pheromone Ostrinia nubilalis (Z strain), and their rationale is reported. Both chemicals exhibited some electrophysiological activity, and topical application
of 10 pg of pheromone analogue on male antennae was sufficient to induce significantly lower depolarization responses to the
pheromone versus untreated insects. In a wind tunnel, the number of European corn borer males attracted to sources containing
mixtures of 1 + pheromone in ratios ≥ 1 :1 was significantly lower than the number attracted to a source containing pheromone alone. Source
contact behaviour was dramatically impaired when the 1 + pheromone blend reached a ratio of 10 :1, in which only 2% of males displayed source contact in the presence of antagonist.
When compound 1 was present at the source, males usually flew upwind with occasional downwind reversals; when compound 2 was present at the lure, males performed wider crosswind reversals, with little progress toward the source. In the field,
traps baited with mixtures of both compounds with the pheromone in ratios of 5 :1 and 10 :1 elicited a significantly decreased
number of male catches. In esterase inhibition assays, compound 2 was a potent inhibitor (IC50 = 70 nM), whereas the non-fluorinated compound 1 was not. The different activity of both compounds is presumed to be due to different mechanisms of action; considerations
for using methyl ketone analogues as new behavioural antagonists of the pheromone are outlined. |
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Keywords: | :" target="_blank">: Ostrinia nubilalis Pyralidae methyl ketones antagonist trifluoromethyl ketones esterase inhibitors |
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