Measuring the antioxidant activity of olive oil mill wastewater using chemiluminescence |
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Authors: | Atanassova Dora Kefalas Panagiotis Psillakis Elefteria |
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Institution: | Laboratory of Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, POBox 85, Chania, 73100 Greece. |
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Abstract: | A sensitive and simple procedure is described for determining the total phenolic/antioxidant levels of olive oil mill wastewater (OMW), using for the first time Co(II)/ethylenediaminetetracetic acid (EDTA)-induced luminol chemiluminescence. Olive oil wastewater samples were tested for their composition in simple phenolic compounds as a function of the extraction system (two- and three-phase centrifugation systems). The results revealed that the three-phase system had a stronger antioxidant activity and a higher total phenolic content than the two-phase system. The relationship between antioxidant values and total phenolic content is also discussed. |
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