| 农杆菌介导将高赖氨酸蛋白基因导入谷秆两用水稻 |
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| 引用本文： | 王逸群,郑金贵,谢宝贵.农杆菌介导将高赖氨酸蛋白基因导入谷秆两用水稻[J].应用与环境生物学报,2005,11(3):276-278. |
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| 作者姓名： | 王逸群 郑金贵 谢宝贵 |
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| 作者单位： | 1. 福建师范大学生物工程学院,福州,350007;福建农林大学生物技术中心,福州,350002
2. 福建农林大学生物技术中心,福州,350002 |
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| 基金项目： | 国家自然科学基金(No.30070467)，福建省科技厅科技项目(2001Z012)，福建省博士后基金项目资助~~ |
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| 摘 要： | 采用农杆菌介导法将高赖氨酸蛋白基因导入到谷秆两用水稻中，GUS组织化学染色、PCR扩增、Southem blot分析表明，该基因已经整合到水稻基因组中，测定9株转基因水稻叶片赖氨酸含量，大部分植株有明显的提高，最高幅度达到了22．71％，图6参15
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| 关 键 词： | 农杆菌 转基因水稻 赖氨酸含量 |
| 修稿时间： | 2004年4月15日 |
TRANSFER OF LYSINE-RICH PROTEIN GENE INTO GRAIN-STRAW-DUAL-USE RICE VIA AGROBACTERIUM TUMEFACIENS |
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| Wang Yiqun,ZHENG Jingui,XIE Baogui.TRANSFER OF LYSINE-RICH PROTEIN GENE INTO GRAIN-STRAW-DUAL-USE RICE VIA AGROBACTERIUM TUMEFACIENS[J].Chinese Journal of Applied and Environmental Biology,2005,11(3):276-278. |
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| Authors: | Wang Yiqun ZHENG Jingui XIE Baogui |
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| Institution: | WANG Yiqun1,2**,ZHENG Jingui2,XIE Baogui2 |
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| Abstract: | Lysine-rich protein gene (lys) was transferred into grain-straw-dual-use rice via Agrobacterium tumefaciens LBA4404 as a vehicle. Histochemical staining for GUS activity, PCR and Southern blot demonstrated that the lysine-rich protein gene was integrated into the genome of the rice plants. Data analysis showed that lysine content in most of 9 transgenic plants was obviously improved, and one of them increased by 22.71 %. Fig 6, Ref 15 |
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| Keywords: | Agrobacterium tumefaciens transgenic rice lysine content |
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