| 不同氮源对海洋卡盾藻生长和硝酸还原酶活性的影响 |
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| 引用本文： | 李斌,欧林坚,吕颂辉.不同氮源对海洋卡盾藻生长和硝酸还原酶活性的影响[J].海洋环境科学,2009,28(3). |
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| 作者姓名： | 李斌 欧林坚 吕颂辉 |
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| 作者单位： | 1. 暨南大学,赤潮与水环境研究中心,广东,广州,510632;暨南大学,环境工程系,广东,广州,510632
2. 暨南大学,赤潮与水环境研究中心,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金-广东省联合基金，国家高技术研究发展计划(863计划)，广东省海洋908专项评价项目 |
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| 摘 要： | 在实验室条件下,研究了不同氮源对海洋卡顿藻(Chattonella marina)生长和藻细胞硝酸还原酶活性(NRA)的影响.结果表明,海洋卡盾藻可以有效利用无机氮源,如NH4Cl、NaNO3、NaNO2,对有机氮源如尿素、甘氨酸和1,4-丁二胺盐酸盐也有一定的利用能力,但不能有效利用丙氨酸.海洋卡顿藻的生长速率与营养盐的同化速率不一致,存在一定的滞后效应,最大比生长率可达到0.7 d-1.藻细胞硝酸还原酶活性在以NaNO3为氮源时,藻细胞酶活性(NRAmax)最大,为20.6 fmol/(min·cell).
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| 关 键 词： | 海洋卡盾藻 氮源 硝酸还原酶 |
Effects of different kinds of nitrogen on growth and nitrate reductase activity of Chattonella marina (Raphidophyceae) |
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| LI Bin,OU Lin-jian,Lv Song-hui.Effects of different kinds of nitrogen on growth and nitrate reductase activity of Chattonella marina (Raphidophyceae)[J].Marine Environmental Science,2009,28(3). |
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| Authors: | LI Bin OU Lin-jian Lv Song-hui |
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| Institution: | 1.Research Center of Harmful Algae and Aquatic Environment;Jinan University;Guangzhou 510632;China;2.Department of Environmental Engineering;China |
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| Abstract: | The growth and nitrate reductase activity(NRA) of Chattonella marina were investigated from the different nitrogen sources.The results showed that C.marina could utilize NH4-N,NO3-N,NO2-N,urea,glycin,and 1,4-diaminobutane hydrochloride as nitrogen sources,but it could not utilize threonine.The growth of C.marina has a lag effect which was not consistent with the assimilation of the nutrients.The maximum growth rate was 0.7 d-1,and NRAmax of C.marina was 20.6 fmol/(min cell),when NO3-N was added as a nitroge... |
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| Keywords: | Chattonella marina nitrate reductase nitrate |
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