| 二噁英对非洲爪蛙细胞色素P450表达的影响 |
| |
| 引用本文： | 董 武,胡玉晖,杨景峰,高原,解彦炯,王思珍,马国文.二噁英对非洲爪蛙细胞色素P450表达的影响[J].环境科学研究,2006,19(1):87-90. |
| |
| 作者姓名： | 董 武 胡玉晖 杨景峰 高原 解彦炯 王思珍 马国文 |
| |
| 作者单位： | 1.内蒙古民族大学,动物科技学院,内蒙古,通辽,028042;酪农学园大学,日本,江别 |
| |
| 基金项目： | 中国科学院资助项目
,
教育部留学回国人员科研启动基金
,
新世纪优秀人才支持计划 |
| |
| 摘 要： | 二噁英(代DD)是一类毒性较强的环境污染物质.试验使用印记法定量检测了非洲爪蛙芳烃基受体(从R)及其核内的转录因子(Amt),细胞色素P450(CYP)中的CYP1A6 mRNA及CYP1A7 mRNA;用免疫反应检测了CYP1A蛋白质的表达.结果表明:TCDD的染毒没有引起AhR和Arnt表达量的增加,但引起CYP1A6 mRNA及CYP1A7 mRNA表达的极显著增加,以及CYP1A蛋白质的强烈表达;TCDD强烈诱发非洲爪蛙CYP1A,且存在质量浓度依存性,使用CYP1A作为环境危险性评价具有一定的可探讨性.
|
| 关 键 词： | 二噁英 细胞色素P450 芳烃基受体 非洲爪蛙 |
| 文章编号： | 1001-6929（2006）01-0087-04 |
| 收稿时间： | 2005-02-10 |
| 修稿时间： | 2005-02-10 |
The Effect of Dioxin in Expression of Cytochrome P450 in Xenopus Embryos |
| |
| DONG Wu,HU Yu-hui,YANG Jing-feng,GAO Yuan,XIE Yan-jiong,WANG Si-zhen and MA Guo-wen.The Effect of Dioxin in Expression of Cytochrome P450 in Xenopus Embryos[J].Research of Environmental Sciences,2006,19(1):87-90. |
| |
| Authors: | DONG Wu HU Yu-hui YANG Jing-feng GAO Yuan XIE Yan-jiong WANG Si-zhen and MA Guo-wen |
| |
| Institution: | 1.Animal Science and Technology College,Inner Mongolia University for nationalists,Tongliao 028042,China;Rakuno Gakuen University,Ebetsu,Japan2.Rakuno Gakuen University,Ebetsu,Japan3.Animal Science and Technology College,Inner Mongolia University for nationalists,Tongliao 028042,China; |
| |
| Abstract: | Dioxin(2,3,7,8-Tetrachlorodibenzo-p-dioxin,TCDD) is a potent environmental pollution.In this experiment,the authors measured aryl hydrocarbon receptor(AhR),Arnt and cytochrome P450(CYP) including CYP1A6 mRNA and CYP1A7 mRNA by northern blot analysis and expression of CYP1A protein by CYP1A anti body in the Xenopus.Increased expression of AhR or Arnt was not observed,but the increased expression of CYP1A6 mRNA and CYP1A7 mRNA and strong expression of CYP1A protein by TCDD was observed.The result suggests that TCDD dose-dependent induced the CYP1A.Maybe CYP1A is useful for environmental risk assessment. |
| |
| Keywords: | TCDD CYP AhR Xenopus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《环境科学研究》浏览原始摘要信息 |
| 点击此处可从《环境科学研究》下载免费的PDF全文 |
|
