| 微生物酶促高效提制废次烟草中烟碱的研究 |
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| 引用本文： | 王星敏,徐龙君,殷钟意,夏晓芳.微生物酶促高效提制废次烟草中烟碱的研究[J].环境工程学报,2010,4(12):2875-2878. |
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| 作者姓名： | 王星敏 徐龙君 殷钟意 夏晓芳 |
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| 作者单位： | 1.重庆大学资源及环境科学学院，重庆 400033; 2.重庆工商大学环境与生物工程学院，重庆 400067；3.重庆工商大学废油资源化技术与装备教育部工程中心，重庆 400067,1.重庆大学资源及环境科学学院，重庆 400033,2.重庆工商大学环境与生物工程学院，重庆 400067；3.重庆工商大学废油资源化技术与装备教育部工程中心，重庆 400067,2.重庆工商大学环境与生物工程学院，重庆 400067 |
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| 基金项目： | 重庆市科技攻关项目资助（cstc，2008bb7253） |
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| 摘 要： | 采用酶促反应利用微生物发酵处理废次烟叶提取烟碱,对比发酵及微波协助等不同方式,利用分光光度法测定发酵体系中烟碱溶出含量,优选出发酵菌种,并探讨接种量和培养基质及不同工艺参数等对烟碱含量的影响。实验结果表明:选用白腐真菌和管囊酵母(1∶1)在微波协助处理下,当烟叶与水的重量比为3∶20,豆粕与烟叶重量百分比为15%,接种量40%,pH=6,30℃发酵48 h,能得到烟草中烟碱溶出浓度为3.291 g/L,为有效实现废次烟草减量化排放与资源化利用结合奠定基础。
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| 关 键 词： | 酶促反应 烟碱 发酵 废次烟草 |
Research on effective extraction of nicotine from tobacco waste by microbial enzyme reaction |
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| Wang Xingmin,Xu Longjun,Yin Zhongyi and Xia Xiaofang.Research on effective extraction of nicotine from tobacco waste by microbial enzyme reaction[J].Techniques and Equipment for Environmental Pollution Control,2010,4(12):2875-2878. |
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| Authors: | Wang Xingmin Xu Longjun Yin Zhongyi and Xia Xiaofang |
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| Institution: | 1. School of Resources and Environmental Science, Chongqing University, Chongqing 400033,China;2. Environmental and Biological Engineering Institute, Chongqing Technology and Business University, Chongqing 400067,China;3.Engineering Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education,Chongqing Technology and Business University, Chongqing 400067, China,1. School of Resources and Environmental Science, Chongqing University, Chongqing 400033,China,2. Environmental and Biological Engineering Institute, Chongqing Technology and Business University, Chongqing 400067,China;3.Engineering Research Centre for Waste Oil Recovery Technology and Equipment, Ministry of Education,Chongqing Technology and Business University, Chongqing 400067, China and 2. Environmental and Biological Engineering Institute, Chongqing Technology and Business University, Chongqing 400067,China |
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| Abstract: | |
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| Keywords: | enzymatic reaction nicotine fermentation tobacco waste |
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