人体呼出气中内源性挥发性有机物筛选研究

人体代谢产生的内源性挥发性有机物（VOCs）可用作健康状况的标志物.本研究选择17~26岁无呼吸系统疾病的高校学生（73人）作为观察对象，使用气相色谱-火焰离子化检测器/质谱联用仪（GC-FID/MS）分析收集到的呼出气样本，对呼出气中VOCs的种类、浓度进行研究，发现了其5种卤代物的浓度高于室内浓度，其次是室外浓度.其中，在人体呼出气中1，1-二氯乙烯的浓度为室内浓度的近20倍，是室外浓度的近300倍，人体呼出气是环境中1，1-二氯乙烯的一个重要源.定量分析健康人呼出气，对比其与环境空气样本的浓度差异，并进行统计学检验，共筛选出1，1二氯乙烯、丙酮、1-己烯、甲基环己烷、2-戊酮、反-2-戊烯、2，4-二甲基戊烷、3-戊酮、四氯乙烯、对二氯苯、间二氯苯11种特征性内源性VOCs.

Screening of endogenous volatile organic compounds in human exhaled breath

Exhaled breath contains thousands of volatile organic compounds (VOCs), and relevant composition could vary with their health status. A cohort of healthy volunteers (73 students without respiratory disease) was recruited in this work. The breath samples were collected using gas-bag and further analyzed by gas chromatography with mass spectrometric detection and flame ionization detector. Five halides in human exhaled breath were found to have higher concentration levels than those of both indoor and outdoor environments. Among them, 1,1-dichloroethylene concentration in human exhaled breath was detected nearly 20times higher than its indoor air concentration, and about 300 times higher than the outdoor one, which suggests that human breath is an important source of 1,1-dichloroethylene in the environment. By quantitative analysis of exhaled breath of recruited healthy people with parallel comparison with indoor counterparts, eleven endogenous VOCs were detected and can be attributed primarily to humans, including 1,1-Dichloroethene, acetone, 1-hexene, methylcyclohexane, 2-pentanone, trans-2-pentene, 2, 4-dimethylpentane, 3-pentanone, C2Cl4, m-dichlorobenzene and p-dichlorobenzene.