微藻生物膜营养环境对微藻生长和油脂积累影响

以琼脂凝胶作为微藻营养物质的供给源层，微孔滤膜作为微藻生物膜吸附基质，构建了源层/基质层双层结构光合微藻生物膜反应器，研究了微藻生物膜营养环境对微藻生长和油脂积累的影响，实验表明：当琼脂浓度从0.125%（W/W）升高到8%时，生物膜含水量从0.62减少到0.25g，微藻细胞直径从3.87减小到3.35μm，微藻生物膜厚度从91.23降低到62.33μm，单位厚度生物膜内含水量降低40.49%，CO₂和光向生物膜内部传递阻力减小，为光合反应提供充足碳源和光照，微藻生物膜密度从40.56提高了45.86%至59.16g/m²，微藻油脂产量提高了63.39%.

Effect of nutrient solution content of biofilm on algal growth and lipid accumulation

Agarose gel were coupled with mixed cellulose (CA-CN) membrane to build a photosynthetic microalgal biofilm reactor containing a two-layer source/substrate structure. The agarose gel layer was used to supply nutrient, while the CA-CN membrane layer was used as the framework for biofilm. The effects of nutrient contents on microalgal growth and lipid accumulation were investigated. As the agar powder content in culture increased from 0.125% to 8% (W/W), the nutrient contents decreased from 0.62 to 0.25g, the cell diameter decreased from 3.87 to 3.35μm, the thickness of microalgal biofilm decreased from 91.23 to 62.33μm, and the water content per unit biofilm thickness reduced by 40.49%. Consequently, the CO₂ and light transfer resistance of internal biofilm decreased, whereas the areal density of microalgal biofilm was improved from 40.56 to 59.16g/m² (45.86% increase), and the lipid production increased by 63.39%.