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解淀粉芽孢杆菌对鱼腥藻的抑藻效果分析与机理初探
引用本文:李超,吴为中,吴伟龙,杨璐华,朱元晴.解淀粉芽孢杆菌对鱼腥藻的抑藻效果分析与机理初探[J].环境科学学报,2011,31(8):1602-1608.
作者姓名:李超  吴为中  吴伟龙  杨璐华  朱元晴
作者单位:北京大学环境科学与工程学院,北京,100871
基金项目:国家自然科学基金面上项目
摘    要:解淀粉芽孢杆菌(Bacillus amyloliquefaciens)在自然界广泛分布,可通过分泌抗生素、抗菌蛋白或多肽类物质起到生物防治作用,但在抑藻方面的研究还处于空白状态.本研究从滇池富营养化水体中分离得到一株溶藻细菌DC1,对该菌的抑藻效果和溶藻机理进行了研究,并通过16SrRNA序列分析方法对其进行种属鉴定....

关 键 词:解淀粉芽孢杆菌  抑藻效果  水华鱼腥藻  溶藻机理
收稿时间:2010/11/5 0:00:00
修稿时间:1/30/2011 3:49:00 PM

Ability of Bacillus amyloliquefaciens to inhibit growth of Anabaena flos-aquae and the inhibition mechanism
LI Chao,WU Weizhong,WU Weilong,YANG Luhua and ZHU Yuanqing.Ability of Bacillus amyloliquefaciens to inhibit growth of Anabaena flos-aquae and the inhibition mechanism[J].Acta Scientiae Circumstantiae,2011,31(8):1602-1608.
Authors:LI Chao  WU Weizhong  WU Weilong  YANG Luhua and ZHU Yuanqing
Institution:College of Environmental Sciences and Engineering, Peking University, Beijing 100871,College of Environmental Sciences and Engineering, Peking University, Beijing 100871,College of Environmental Sciences and Engineering, Peking University, Beijing 100871,College of Environmental Sciences and Engineering, Peking University, Beijing 100871 and College of Environmental Sciences and Engineering, Peking University, Beijing 100871
Abstract:An algae-lysing bacterium strain DC1 was isolated from Dianchi Lake and identified as Bacillus amyloliquefaciens using 16S rDNA sequence analysis technique. It was widely distributed in natural environment. This bacterium could produce antibiotics, antibacterial protein and polypeptide to achieve biological control. However, its algae-lysing ability has not been reported. In this study, the algae-inhibiting effect and algicidal mechanism were investigated. The results showed that DC1 has especially good effects on lysing Anabaena flos-aquae. When the DC1 density was 108 cells•mL-1 and the initial concentration of Bacillus amyloliquefaciens ranged from 0.23 ~1.92 mg•L-1, the average removal rate would reach 95.54% - 91.35%, averagely 93.5% after 6 days. The density of the DC1 and Bacillus amyloliquefaciens were related with the removal rate. Some extracellular substance produced by this strain had the algae-lysing ability, and it is thermo-stable and non-protein substance.
Keywords:Bacillus amyloliquefaciens  algae-inhibition effect  Anabaena flos-aquae  algae- lysing mechanism
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