正交实验选择嗜碱细菌降解木质素的金属离子最优综合培养条件

本文在复合碳源的碱性液体培养条件下 (p H≈ 10 .5 ) ,用正交实验法对嗜碱木质素降解细菌 6号菌株降解木质素的金属离子培养条件进行了优化。结果表明 ,各金属离子对 6号菌株产生的木质素降解酶 L ac-case和 Mn P以及木质素降解率都有一定促进作用。分析正交实验结果表明 ,其中 Mn2 +和 Cu2 +是最主要的影响因素 ,各种金属离子的最佳综合水平为 :Mn SO4 · H2 O0 .7g/ L、Cu SO4 · 5 H2 O1m g/ L、Fe SO4 · 7H2 O5 mg/L、Zn SO4 · 7H2 O 30 m g/ L、Ca Cl2 · 2 H2 O 5 0 m g/

Optimistical synthetic culture condition of five metal-ion on lignin biodegradation ability of alkaliphilic ligninolytic bacteria using orthogonal experimental design

The effects of metal ion Mn 2+ ?Cu 2+ ?Fe 2+ ?Zn 2+ and Ca 2+ on lignin biodegradation ability of alkaliphilic ligninolytic bacteria strain 6 in alkaline liquid medium (pH≈10.5) with compounded carbons are optimized using orthogonal experiments. The result show the five metal-ions promote the activities of Laccase and MnP and the degradation rate of wheat straw lignin. The synthetic result is analysised. Mn 2+ and Cu 2+ are the key factors. The optimal synthetic condition can take MnSO 4·H 2O 0.7g/L?CuSO 4·5H 2O 1mg/L?FeSO 4·7H 2O 5mg/L?ZnSO 4·7H 2O 30mg/L and CaCl 2·2H 2O 50mg/L.