水体铜对黄河鲤Na+-K+-ATP酶活性的影响

为了研究不同浓度的水体铜对黄河鲤Na -K -ATP酶活性的影响,采用ρ(Cu2 )为0.01,0.05,0.10,0.30,0.50,0.70和1.00 mg/L的试验液分别刺激黄河鲤1,3,5和7 d后,测定黄河鲤鳃组织、肝胰脏和肾组织Na -K -ATP酶活性.结果表明:Cu2 对黄河鲤的96 h LC50为1.67 mg/L;低浓度Cu2 (ρ(Cu2 )为0.01和0.05 mg/L)对黄河鲤鳃组织、肝胰脏和肾组织Na -K -ATP酶活性有促进作用,且随着ρ(Cu2 )的增加和暴露时间的延长,促进作用也越明显(P<0.05);黄河鲤暴露于0.10 mg/L的Cu2 溶液时,鳃组织Na -K -ATP酶活性5 d后显著低于对照组(P<0.05),肝胰脏和肾组织Na -K -ATP酶活性与对照组相比,差异不显著;高浓度Cu2 (ρ(Cu2 )为0.30,0.50,0.70和1.00 mg/L)对黄河鲤鳃组织、肝胰脏和肾组织Na -K -ATP酶活性有抑制作用,且随着ρ(Cu2 )的增加和暴露时间的延长,抑制作用也越明显(P<0.05或P<0.01).提示黄河鲤鳃组织、肝胰脏和肾组织Na -K -ATP酶活性对铜的污染均具有指示作用(其中最为灵敏的是鳃组织Na -K -ATP酶活性),且存在计量-效应关系和时间-效应关系,可以用来指示低剂量重金属的污染.

Effects of Copper on the Na+-K+-ATPase Activities of Cyprinus Carpio

Mirror Cyprinus carpio were exposed to different concentrations of copper such as 0.01, 0.05, 0.10, 0.30, 0.50, 0.70 and 1.00 mg/L. The Na⁺-K⁺-ATPase activities in different tissue such as gill, liver and kidney were determined after 1 day, 3 days, 5 days and 7 days in order to study the effects of copper on the Na⁺-K⁺-ATPase activities of Cyprinus carpio. The results showed that, when exposed to the lower concentrations such as 0.01 and 0.05 mg/L, the Na⁺-K⁺-ATPase activities in three tissues were promoted. Thehigher the concentration of copper or the longer the exposure time was, the more the promotive effects was enhanced (P<0.05). When exposed to the concentrations of 0.10 mg/L, the Na⁺-K⁺-ATPase activities in gill tissue weresignificantly lower than those of the control after 5 days (P<0.05), but theNa⁺-K⁺-ATPase activities in liver and kidney tissue were no significantlydifferent compared with those of the control. When exposed to the higher concentrations such as 0.30, 0.50, 0.70 and 1.00 mg/L, the Na⁺-K⁺-ATPase activities in three tissues were inhibited. The higher the concentration of copper or the longer the exposure time was, the more the inhibiting effects was enhanced (P<0.05 or P<0.01). These results indicated that there were indicative functions for the copper contamination of the Na⁺-K⁺-ATPase activities inthe three tissues such as gill, liver and kidney of Cyprinus carpio (the Na⁺-K⁺-ATPase activities in gill tissue were the most sensitive), and showedobvious dose-effect and time-effect response relationship. They could be used as a biomarker in evaluating heavy metal contamination.