反胶束体系中漆酶催化愈创木酚的研究

采用4种不同属性类型的表面活性剂(CTAB,Tween-80, AOT以及鼠李糖脂)构建反胶束体系,以木质素模式物愈创木酚为底物,研究了漆酶在反胶束环境中催化愈创木酚的特性,通过对不同反胶束环境中漆酶酶活的对比,确定在4种反胶束体系中,适合酶解反应的最佳表面活性剂浓度和对应的最佳含水率w0.分别为:CTAB浓度20mmol/L,w0=20;Tween 80浓度50mmol/L,w0=25;AOT浓度30mmol/L,w0=10;鼠李糖脂浓度10mmol/L,w0=30.并考察了表面活性剂类型属性对愈创木酚在反胶束体系中酶解行为的影响和底物动力学.结果表明,最佳表面活性剂浓度以及对应的最适含水率条件下,阴离子表面活性剂AOT和鼠李糖脂的反胶束体系中愈创木酚底物的催化效果较好,鼠李糖脂反胶束体系中底物的催化率最高,反应6h后分别比 CTAB、Tween-80以及AOT反胶束体系中的转化率高44.12%、34.6%、29.43%.

Guaiacol catalysis by laccase in the reverse micellar systems

In order to investigate the characteristics of the catalytic activity of laccase, several types of surfactants were adopted to construct reverse micelles, using guaiacol as substrate. The activity of laccase activity was measured to determine the optimum surfactant concentration and corresponding water solubilization w0 of CTAB, Tween-80, AOT and rhamnolipid reverse micelle systems. The optimum surfactant concentrations were 20, 50, 30, 10mmol/L with corresponding water content of 20, 25, 10, 30mmol/L for CTAB, Tween-80, AOT and rhamnolipid reverse micellar systems, respectively. Under this condition the effects of surfactant type on the catalytic activity of reverse micellar systems were studied. The transforming rate of substrate in the micellar system constructed by anionic surfactant was higher than these of the others, and the rhamnolipid micelle system performed best. After 6h, the conversion rate of rhamnolipid reverse micellar systems compared with CTAB,Tween-80and AOT reverse micelle systems were 44.12%, 34.6% and 29.43% higher, respectively.