利用高通量测序对封存CO2泄漏情景下土壤细菌的研究

全球CO2浓度不断升高的背景下,地质封存CO2为控制大气CO2浓度上升提供了全新的思路,但封存CO2潜在的泄漏风险也对生态系统产生重大威胁.土壤微生物与土壤健康密切联系.鉴于目前地质封存CO2泄漏对土壤微生物影响的研究鲜有报道,本研究通过模拟地质封存CO2泄漏情景,利用Miseq平台Illumina第二代高通量测序土壤细菌16S rRNA基因,结合相关生物信息学分析,初步探讨了封存CO2泄漏情景下农田土壤细菌群落丰富度、多样性和群落结构的变化.试验共获得15个土壤样本的43 017个OTU共486 645条读数,生物学分析结果表明,封存CO2泄漏通量和时间不同的情景下,农田土壤细菌丰富度和细菌群落多样性的变化存在差异,初步推测CO2泄漏量增大和泄漏时间持续更长的情景下,土壤细菌丰富度和多样性下降幅度较大,土壤细菌群落中优势菌群向若干细菌集中,其中土壤酸杆菌门细菌相对增多可能作为地质封存CO2泄漏对土壤生态系统影响的生物监测指标.

Research on Soil Bacteria Under the Impact of Sealed CO2 Leakage by High-throughput Sequencing Technology

Carbon dioxide Capture and Storage has provided a new option for mitigating global anthropogenic CO₂ emission with its unique advantages. However, there is a risk of the sealed CO₂ leakage, bringing a serious threat to the ecology system. It is widely known that soil microorganisms are closely related to soil health, while the study on the impact of sequestered CO₂ leakage on soil microorganisms is quite deficient. In this study, the leakage scenarios of sealed CO₂ were constructed and the 16S rRNA genes of soil bacteria were sequenced by Illumina high-throughput sequencing technology on Miseq platform, and related biological analysis was conducted to explore the changes of soil bacterial abundance, diversity and structure. There were 486645 reads for 43017 OTUs of 15 soil samples and the results of biological analysis showed that there were differences in the abundance, diversity and community structure of soil bacterial community under different CO₂ leakage scenarios while the abundance and diversity of the bacterial community declined with the amplification of CO₂ leakage quantity and leakage time, and some bacteria species became the dominant bacteria species in the bacteria community, therefore the increase of Acidobacteria species would be a biological indicator for the impact of sealed CO₂ leakage on soil ecology system.