Cellulose degrading capabilities of cellulolytic bacteria isolated from the intestinal fluids of the silver cricket

Cellulose degrading bacteria were isolated from the intestinal fluid of the silver cricket Lepisma Sp. and a culture was developed anoxigenically in the cellulose degrading medium following standard laboratory techniques. The cellulolytic activity of the microbe was examined in a broth culture using Whatman 42 filter paper as the source of insoluble cellulose. Activity was measured spectrophotometrically (620nm) following Anthrone reaction of the culture filtrate and the sugar produced was quantified as a factor of time. The pH optimum was found to be between 7 and 8. Temperature profile optimum was between 30–37°C.The microbes appeared as white colonies on a solid medium. Morphologically the bacterium is a gram-positive nonspore forming rod which was tentatively identified as a new strain of Cellulomonas sp.