| Heavy metal induced DNA changes in aquatic macrophytes： Random amplified polymorphic DNA analysis and identification of sequence characterized amplified region marker |
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| 作者姓名： | Meetu Gupt Neera Bhalla Sarin |
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| 作者单位： | Meetu Gupta(Department of Biosciences, Jamia Millia Islamia, New Delhi-110025, India);Neera Bhalla Sarin(School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India)
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| 基金项目： | supported financially by Department of Science and Technology, New Delhi, India under the SERC-DST fasttrack scheme. |
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| 摘 要： | Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10 μmol/L Cd, 5 μmol/L Hg, and 20 μmol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the ...
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| 关 键 词： | 随机扩增多态性DNA分析 水生植物 序列特征 金属诱导 DNA图谱 识别 蛋白质含量 稳定性试验 |
| 收稿时间： | 6/2/2008 12:00:00 AM |
| 修稿时间： | 14 July 2008 |
Heavy metal induced DNA changes in aquatic macrophytes: Random
amplified polymorphic DNA analysis and identification of sequence
characterized amplified region marker |
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| Meetu Gupt,Neera Bhalla Sarin.Heavy metal induced DNA changes in aquatic macrophytes: Random
amplified polymorphic DNA analysis and identification of sequence
characterized amplified region marker[J].Journal of Environmental Sciences,2009,21(5):686-690. |
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| Authors: | Meetu Gupta and Neera Bhalla Sarin |
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| Institution: | 1. Department of Biosciences, Jamia Millia Islamia, New Delhi-110025, India
2. School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India |
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| Abstract: | Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10 mol/L Cd, 5 mol/L Hg, and 20 mol/L Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA (RAPD) profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region (SCAR) marker is useful in detecting the effects of genotoxin agents. |
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| Keywords: | RAPD Hydrilla verticillata Ceratophyllum demersum DNA polymorphism SCAR marker |
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