CuSO4对松江鲈肾细胞系的毒性效应

本文研究了不同浓度的CuSO₄对55-65代松江鲈肾细胞系的毒性效应。采用MTT法测得CuSO₄ 24 h LC₅₀为154.34 μmol/L。酶活力测定的结果显示:超氧化物歧化酶（SOD）和谷胱甘肽-S-转移酶（GST）的活性在CuSO₄浓度为0~300 μmol/L时，随浓度的升高逐渐升高，在300 μmol/L时达到最大值；谷胱甘肽过氧化物酶（GSH-Px）的活性在CuSO₄浓度为100 μmol/L时达到最大值，随后随着CuSO₄浓度的降低逐渐降低。微核率随CuSO₄浓度增加而增加，最高达14.33‰，彗星实验发现在半致死浓度条件下松江鲈肾细胞拖尾率为54.00%、迁移长度18.41±2.94 μm，与对照组差异显著（p ＜0.05）。认为CuSO₄会引起细胞氧化酶活性的改变以及细胞核DNA损伤。松江鲈肾细胞系可以作为Cu2+污染的监测指标。

Cytotoxicity studies on cupric sulfate to roughskin sculpin kidney fin cell lines in vitro

In this study, kidney cell line from trachidermus fasciatus were used to assess toxicity of cupric sulfate in vitro.The 24 h semi-lethal concentrations of kidney cell measured by Thiazole blue (MTT) method were 154.34 μmol/L.After exposure of cupric sulfate, when the cupric sulfate concentration was 0~300 μmol/L, the activities of superoxide dismuta (SOD) and glutathione S-transferase (GST) in kidney cell line were increased with the increase of concentration, while the concentration was 300~400 μmol/L, the activities of both enzymes gradually decreased.When the concentration was 100~300 μmol/L, the activity of Glutathione peroxidase (GSH-Px) in kidney cell line was negatively correlated with the concentration of cupric sulfate.Micronucleus test showed that cupric sulfate could cause the nucleus damage and form micronucleus.The rate of micronucleus increased with the increase of cupric sulfate concentration.the maximum micronucleus rates of kidney cell line were 14.33‰.Comet assay showed that cupric sulfate could cause the nucleus damage.The comet rate were 54.00% and comet length of liver DNA were 18.41±2.94 μm.The comet rate and comet length of kidney cell DNA treated by cupric sulfate in test groups increased significantly (p < 0.05) compared with control group.The kidney cell line from trachidermus fasciatus can be as monitoring marker of copper pollution.