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山西高原油松种群遗传多样性
引用本文:李毳,柴宝峰,王孟本. 山西高原油松种群遗传多样性[J]. 生态环境, 2005, 14(5): 719-722
作者姓名:李毳  柴宝峰  王孟本
作者单位:1. 山西大学黄土高原研究所,山西,太原,030006;山西财经大学环境经济系,山西,太原,030006
2. 山西大学黄土高原研究所,山西,太原,030006
基金项目:国家自然科学基金项目(30170150);山西省自然科学基金项目(20041079)
摘    要:用酸性聚丙烯酰胺凝胶电泳(A-PAGE)技术,分析了山西高原9个油松种群在醇溶蛋白水平上的遗传多样性。135份材料共分离出23条带,其中3条为共有带,多态性高达86.95%。全部材料共出现53种带型,9个不同油松种群的带型有差异,同一种群不同个体的带型也有所不同,说明山西高原的油松在遗传上已产生一定程度分化,在醇溶蛋白水平上呈现出遗传多态性。从供试材料的带型计算出油松遗传分化系数为0.1547。即在种群间的变异占总变异的15.47%,种群内变异为84.53%,大部分的遗传变异存在于种群内,但种群间的分化程度在松属树种中也属于较高水平。根据23个多态位点计算遗传相似系数和遗传距离,进行聚类分析,将山西高原9个油松种群聚为3个类群。

关 键 词:山西高原 油松 醇溶蛋白 遗传多态性 聚类分析
文章编号:1672-2175(2005)05-0719-04
收稿时间:2005-05-13
修稿时间:2005-05-13

Genetic diversity of populations of Chinese pine (Pinus tabulaeformis Carr.) in Shanxi plateau
LI Cui,CHAI Bao-feng,WANG Meng-ben. Genetic diversity of populations of Chinese pine (Pinus tabulaeformis Carr.) in Shanxi plateau[J]. Ecology and Environmnet, 2005, 14(5): 719-722
Authors:LI Cui  CHAI Bao-feng  WANG Meng-ben
Abstract:Genetic diversity of prolamins in 135 samples of 9 populations of Chinese pine(Pinus tabulaeformis) in Shanxi Plateau was investigated by using acidic polyacrylamide gel electrophoresis(A-PAGE) technique.A total of 23 bands were identified,in which 3 bands were common bands.The percentage of polymorphic loci reached up to 86.95%.The differentiation coefficient of Pinus populations was 0.154 7,which was calculated from the band types of prolamins of samples.Variations in inter-population account for 15.47% in general variations,while variation in intra-population account for 84.53% in general variation.Most of genetic variation occur in intra-population,however the level of genetic differentiation in inter-population of Pinus is relative high.The re-sults of clustering analysis using the genetic similarity coefficient of 23 polymorphic loci indicated that 9 populations of Chinese pine could be divided into three groups in the point of genetic distance 1.114 0.
Keywords:Shanxi plateau   Pinus tabulaeformis   prolamin   genetic diversity   clustering analysis
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