Flow cytometric method to measure urea-resistant alkaline phosphatase from blood neutrophils for use in the prenatal screening of Down's syndrome |
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| Authors: | Dr Yash P Agrawal Päivi Ylikangas Markku T Parviainen Ilkka M Penttilä |
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| Institution: | 1. Department of Clinical Chemistry, Kuopio University Hospital, POB 1777, 70211 Kuopio, Finland;2. A. I. Virtanen Institute, Kuopio, Finland |
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| Abstract: | The histochemical measurement of urea-resistant alkaline phosphatase from maternal blood neutrophils is known to have a high detection rate for the prenatal detection of Down's syndrome pregnancies. However, because the histochemical method is laborious and subjective to use, it has not gained widespread acceptance in prenatal screening programmes. We present a simple and objective method for the measurement of urea-resistant alkaline phosphatase by flow cytometry. The method should allow the design of larger studies aimed at evaluating the role of neutrophil urea-resistant alkaline phosphatase in the prenatal screening for Down's syndrome. |
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| Keywords: | Flow cytometry Down's syndrome alkaline phosphatase prenatal screening |
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