以葡萄糖为共基质硝基酚厌氧生物降解性试验研究

在35℃中温厌氧条件下,以葡萄糖为共基质,采用间歇试验法,通过测定甲烷累积产量,研究了2-硝基酚、4-硝基酚、2,4-二硝基酚和2,6-二硝基酚的厌氧生物降解性,利用相对活性值来判断硝基酚对产甲烷菌的抑制程度.研究结果表明:2-硝基酚浓度小于24mg/L时,对产甲烷菌没有产生抑制作用;4-硝基酚浓度小于20mg/L时,对产甲烷菌没有产生抑制作用,浓度为24mg/L时产生轻度抑制;2,6-二硝基酚浓度小于12mg/L时未产生抑制作用,浓度为16mg/L～24mg/L时产生轻度抑制;2,4-二硝基酚浓度小于4mg/L时没有产生抑制作用,浓度为8mg/L～24mg/L时产生中度抑制.4种物质对产甲烷菌活性的抑制由小到大的排列顺序为:2-硝基酚＜4-硝基酚＜2,6-二硝基酚＜2,4-二硝基酚.

Anaerobic Biodegradation of Nitrophenols with Glucose as Co-substrate

A batch anaerobic test was conducted to examine the biodegradation of 2-nitrophenol, 4-nitrophenol, 2,4-dinitrophenol and 2,6-dinitrophenol through measuring accumulative methane production. The Relative activity values were used to judge the inhibition level of nitrophenols on methanogenic bacteria. The test conditions was as follow: glucose was used as co-substrate and the temperature is 35 degrees C. It didn't cause inhibition when concentrations of 2-nitrophenol, 4-nitrophenol, 2,6-dinitrophenol and 2,4-dinitrophenol were below 24mg/L, 20mg/L, 12mg/L and 4mg/L respectively. Slight inhibition was caused when concentrations of 4-nitrophenol and 2,6-dinitrophenol were 24mg/L and 16mg/L-24mg/L respectively. Middle inhibition when concentrations of 2,4-dinitrophenol were 8mg/L-24mg/L was observed. The inhibition level was 2,4-dinitrophenol > 2,6-dinitrophenol > 4-nitrophenol > 2-nitrophenol.