产碱菌株F-3-4降解2,6-二叔丁基苯酚机理研究

研究有毒化合物的生物降解途径．以鉴定有毒化合物在微生物作用下的降解中间产物．以及是否最终成为无害的产物（CO2和H2O），对人们认识有毒化合物在环境中的迁移规律及生物去除的办法．途径具有重要的启示作用。通过色质联机测试中间产物以及降解过程体系酶的变化，对产碱菌株F-3-4降解2,6-二叔丁基苯酚（2,6-DTBP）的途径和机理进行了研究。GC—MS测试结果表明。中间降解产物为乙酸、丙酮酸和异丁酸等化合物。降解过程体系酶测定结果显示．儿茶酚2，3-双加氧酶变化比较明显。表明在菌种生物降解底物过程中。主要以2，3位开裂为主。即主要以间位开裂途径进行开环反应，从而推测了F-3-4对2,6-DTBP的降解途径和机理。

A Study on Metabolic Pathways of 2,6-di-tert-butylphenol Degradation by Alcaligenes F-3-4

The degradation of 2,6-di-tert-butylphenol (2,6-DTBP) by aerobic bacterial strain Alcaligenes F-3- 4 was studied. Intermediate products of the degradation and site of primary attack on the benzene skeleton were investigated. The GC-MS result showed that the intermediate products were acetic acid,pyruvic acid,isobutyric acid,and other compounds. Identified metabolites and catabolic enzymes illustrated that the used bacterial strain attacked the benzene skeleton at the 2,3-position to demethylate 2,6-DTBP. Metabolic pathways of the degradation of 2,6-DTBP were thus proposed.