Evaluation of 3-hydroxy-benzo[a]pyrene levels in Nile tilapia (Oreochromis niloticus) after waterborne exposure to Benzo[a]pyrene |
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| Authors: | Ledicia Rey-Salgueiro Joana Costa Marta Ferreira Maria Armanda Reis-Henriques |
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| Institution: | 1. CIIMAR/CIMAR – Interdisciplinary Centre of Marine and Environmental Research, Laboratory of Environmental Toxicology, University of Porto , Rua dos Bragas, 289, 4050-123, Porto, Portugal lrey@ciimar.up.pt;3. CIIMAR/CIMAR – Interdisciplinary Centre of Marine and Environmental Research, Laboratory of Environmental Toxicology, University of Porto , Rua dos Bragas, 289, 4050-123, Porto, Portugal;4. ICBAS/UP – Institute of Biomedical Sciences Abel Salazar, University of Porto , Largo Professor Abel Salazar, 2, 4099-003 Porto, Portugal;5. CIIMAR/CIMAR – Interdisciplinary Centre of Marine and Environmental Research, Laboratory of Environmental Toxicology, University of Porto , Rua dos Bragas, 289, 4050-123, Porto, Portugal |
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| Abstract: | 3-Hydroxy-benzoa]pyrene (3-OH-BaP), a toxic compound with the ability to covalently bind with the macromolecules (proteins and DNA), is one of the major phenolic metabolites of benzoa]pyrene (BaP). The purpose of this study was to evaluate the presence of 3-OH-BaP in the bile and plasma of Nile tilapia by HPLC with fluorescence detection, after waterborne exposure to BaP (10 and 100?µg?L?1). Metabolites were detected in bile and plasma, and conjugates of 3-OH-BaP (glucuronide and/or sulphate conjugates) were the majority forms in both biological fluids, glucuronide 3-OH-BaP being the main metabolite in bile. Our data suggest that extrahepatic tissues as intestine or gill are important in BaP metabolism and need to be the considered sources of metabolites released into the blood. Although, low levels of 3-OH-BaP in toxic form (free form) were detected in plasma, one cannot exclude the possibility of circulating the levels leading to adverse effects. |
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| Keywords: | PAHs metabolites plasma fish bile HPLC-fluorescence |
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