Preliminary shotgun glycoproteome analysis of N-glycosylation sites in serum proteins of ricin-intoxicated rats |
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| Authors: | Peng Liao Yunhu Li Wensen Liu |
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| Affiliation: | 1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, P. R. China;2. Institute of Military Veterinary Science, Academy of Military Medical Science, Changchun, P. R. Chinaliaopeng@isa.ac.cn;4. Hunan Biological and Electromechanical Polytechnic, The Party and Government Office, Changsha, P. R. China;5. Institute of Military Veterinary Science, Academy of Military Medical Science, Changchun, P. R. China |
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| Abstract: | In this study, the variations of serum glycoproteins after exposure to ricin toxin (RT) in Wistar albino rats are reported. For glycopeptide enrichment, Microcon YM-3 centrifugal filter device capture and micro-liquid chromatography (LC)–electrospray ionization (ESI)–tandem mass spectrometric (MS/MS) analysis were used; 74 trypsin-digested proteins were identified in the control group, and 58 in the RT-intoxication group. Additionally, 33 N-glycosides and 14 glycoproteins were identified in the control, and 50 and 21 in the RT-intoxication group. |
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| Keywords: | ricin toxin (RT) serum proteins N-glycosylation glycoproteome linear trap quadruple (LTQ)-Orbitrap multiple organ dysfunction syndromes (MODS) |
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