Comparison of basal cytotoxicity of seven carbamates in CHO-K1 cells |
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| Authors: | María José Ruiz Lina E Festila Mónica Fernández |
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| Institution: | 1. Laboratori de Toxicologia, Facultat de Farmàcia , Universitat de València , Burjassot, Valencia, Spain M.Jose.Ruiz@uv.es;3. Laboratori de Toxicologia, Facultat de Farmàcia , Universitat de València , Burjassot, Valencia, Spain |
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| Abstract: | The cytotoxic effects of seven carbamate pesticides, aldicarb, aldicarb sulfone, aldicarb sulfoxide, benfuracarb, pirimicarb, propoxur and thiobencarb, were compared in Chinese hamster ovary (CHO-K1) cell line of Circetulus griseus. The endpoints evaluated were lysosomal function by neutral red assay and mitochondrial integrity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay). The carbamates tested were evaluated in both serum-free medium and in serum-containing medium. Results demonstrate that CHO-K1 lysosomes appeared more susceptible to propoxur, aldicarb and its metabolites than mitochondria. Aldicarb was the most toxic carbamate pesticide tested on CHO-K1 cells and aldicarb sulfoxide the least. The presence of foetal calf serum (FCS) diminished significantly the cytotoxicity of tested carbamates. |
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| Keywords: | Carbamates CHO-K1 cells basal cytotoxicity |
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