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Single walled carbon nanotubes induce cytotoxicity and oxidative stress in HEK293 cells
Authors:A. Rama Narsimha Reddy  D.R. Krishna  V. Himabindu
Affiliation:1. Department of Pharmacology and Toxicology, Vaageswari College of Pharmacy, Karimnagar, Telangana, India;2. Department of Pharmacology, Kakatiya University, Warangal, India;3. Covidien/Mallinckrodt Inc., St. Louis, MO, USA;4. Institute of Science and Technology, Centre for Environment, JNTU, Hyderabad, India
Abstract:The aim of the present study was to evaluate the potential toxicity and general mechanisms involved in single walled carbon nanotubes (SWCNTs)-induced cytotoxicity using human embryonic kidney cell line (HEK293) cells. Carbon nanotubes (coded as CNT) used in this study were synthesized by the chemical vapor deposition method. To elucidate the possible mechanisms underlying SWCNT-induced cytotoxicity, cell viability, cell membrane damage (lactate dehydrogenase activity (LDH) assay), reduced glutathione (GSH), interleukin-8 (IL-8) and lipid peroxidation products levels were quantitatively assessed following SWCNT exposure for 48 hr using HEK293 cells. Exposure of cells to SWCNT at 3–300 μg/ml produced significant reduction in cell viability in a concentration-dependent manner. The IC50 value of SWCNT was found to be 87.58 μg/ml. Exposure of HEK cells to SWCNT at 10–100 μg/ml resulted in concentration-dependent cell membrane damage, increased production of IL-8, elevated levels of thiobarbituric acid reactive substances like malondialdehyde and decreased intracellular GSH levels. In summary, exposure to SWCNT resulted in a concentration-dependent cytotoxicity in cultured HEK293 cells that was associated with increased oxidative stress.
Keywords:single walled carbon nanotubes  SWCNT  HEK293 cells  oxidative stress  cytotoxicity  in vitro
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