Genotoxic assessment of anthracene and benzo [a] pyrene to milkfish Chanos chanos |
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| Authors: | L Palanikumar AK Kumaraguru CM Ramakritinan M Anand |
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| Institution: | 1. Department of Marine and Coastal Studies , School of Energy, Environment and Natural Resources, Madurai Kamaraj University , Madurai – 625 021 , Tamil Nadu , India palanikumarl@gmail.com;3. Department of Marine and Coastal Studies , School of Energy, Environment and Natural Resources, Madurai Kamaraj University , Madurai – 625 021 , Tamil Nadu , India |
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| Abstract: | Mutagenic and genotoxic effects of polycyclic aromatic hydrocarbons, anthracene and benzo a] pyrene (BaP), in milkfish Chanos chanos were determined using micronucleus (MN) test and comet assay (CA). Distinct mean frequencies of nuclear abnormalities such as MNs; binucleated micronuclei, nuclear bud, and fragmented apoptotic cells were measured. Significant increase in DNA damage with five classes of damage level was observed and expressed in terms of arbitrary unit (AU). Mean frequencies of total nuclear abnormalities were 0.5?±?0.25 cells in control; 0.67?±?0.33 cells in solvent control; 70?±?9.60 cells in 0.176?mg?L?1 anthracene, and 91.83?±?6.25 cells in 0.031?mg?L?1 BaP. The greatest DNA damage of 170AU was observed in 0.176?mg?L?1 anthracene-exposed group and 182AU was observed in 0.031?mg?L?1 BaP-treated fish. This study confirmed that the CA and MN assays are useful tools in determining potential genotoxicity of water pollutants and might be appropriate as a part of monitoring program. |
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| Keywords: | anthracene benzo [a] pyrene Chanos chanos comet assay DNA damage and micronucleus test |
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