Screening of endogenous antioxidants in some medicinal plants |
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Authors: | RK Choudhary Ajaya Eesha Saroha PL Swarnkar |
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Institution: | 1. Department of Botany , University of Rajasthan , Jaipur 302 004, India ramachoudhary@rediffmail.com;3. Department of Botany , University of Rajasthan , Jaipur 302 004, India |
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Abstract: | The evaluation of antioxidant enzymes and antioxidant metabolites was done in leaf tissues of Azadirachta indica, Butea monosperma, Cassia fistula, Mangifera indica, and Syzygium cumini growing in the Thar Desert, Rajasthan, India. The plants are naturally exposed to drought stress and high temperatures during summer. Enzymatic reactive oxygen species (ROS) scavenging mechanisms in plants include superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase, catalase, and guaiacol peroxidase, and non-enzymatic antioxidants including the carotenoids, proline, and vitamin C were studied. The strategies to cope up with ROS under these extreme conditions are plant-specific. The highest activity of APX was found in M. indica (13.6?±?2.4?units?g?1 fresh wt.). A. indica exhibited maximum guaiacol peroxidase activity (0.024?±?0.006?units?min?g?1 fresh wt.), while S. cumini showed maximum SOD (12.5?±?2.3?units?g?1 fresh wt.) and catalase activities (6.9?±?2.2?units?g?1 fresh wt.). M. indica and S. cumini have been found to be more potent antioxidant systems among the studied plants. |
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Keywords: | antioxidant enzymes antioxidant metabolites reactive oxygen species proline drought stress temperature stress |
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