Lead distribution in rats following respiratory exposure to lead-contaminated soils |
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| Authors: | Genevieve M. Fent Timothy J. Evans Desmond I. Bannon Stan W. Casteel |
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| Affiliation: | 1. Veterinary Medical Diagnostic Laboratory , University of Missouri, Columbia, MO, USA fentg@missouri.edu;3. Veterinary Medical Diagnostic Laboratory , University of Missouri, Columbia, MO, USA;4. U.S. Army Center for Health Promotion &5. Preventive Medicine , Aberdeen Proving Ground, MD, USA |
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| Abstract: | Two preliminary experiments were performed to explore the use of an intratracheal instillation technique as a cost-effective method of determining the biokinetics of lead (Pb) following respiratory exposure to Pb-contaminated soils. A novel intratracheal instillation procedure was refined and used to deliver a defined dose of Pb-contaminated soil or PbAc to the lower respiratory tract of rats. In the first experiment, rats were sacrificed at numerous time intervals post-dosing, and liver, kidney, blood, and bone tissues were collected for Pb analysis. In the second experiment, rats were dosed with Pb-contaminated soil or PbAc via intratracheal instillation or gastric gavage. All rats were sacrificed 96 h after dose administration and tissues were collected for lead analysis. Data from these experiments indicate Pb is well-absorbed following intratracheal instillation of Pb-contaminated soil, and the intratracheal instillation technique could be used as a cost effective method for exploring the biokinetics of Pb in Pb-contaminated soils following respiratory tract exposure. |
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| Keywords: | intratracheal instillation lead soil |
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