Reduction of hexavalent chromium using fungi and bacteria isolated from contaminated soil and water samples |
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| Authors: | Reuel M Bennett Paul Rodrigo F Cordero Gershon S Bautista |
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| Institution: | 1. Faculty of Graduate School, Laboratory of Pure and Applied Microbiology-Research Center for the Natural and Applied Sciences , University of Santo Tomas , Manila , Philippines;2. Department of Biology , College of Arts, Sciences and Education, St. Paul University , Quezon City , Philippines;3. Faculty of Graduate School, Laboratory of Pure and Applied Microbiology-Research Center for the Natural and Applied Sciences , University of Santo Tomas , Manila , Philippines |
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| Abstract: | The reductive adsorption of hexavalent chromium (Cr6+) using six indigenous microorganisms isolated from contaminated soil and water samples was investigated. Quantification of Cr6+ reduction was determined using the 1,5-diphenylcarbazide method followed measuring the absorbance at OD540. Bacterial isolates identified as Klebsiella pneumoniae, Bacillus firmus and Mycobacterium sp. were capable of absorbing Cr6+ efficiently into their biomass, whereas the fungal isolates, Aspergillus flavus, Aspergillus sp. and A. niger were capable of transforming Cr6+ to Cr3+ relative to cell-wall-binding properties. Infrared spectral analysis of functional groups showed that ?OH, ?NH2 and C?O with conjugated ?NH were the binding groups responsible for adsorption of Cr6+ within the biomass of isolates. The data highlight the promising biotechnological application of these isolates in removing carcinogenic and mutagenic Cr6+ from contaminated ecosystems. |
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| Keywords: | Cr6+ Cr3+ bioremediation indigenous microorganisms biotransformation bioreduction bioabsorption |
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