| Abstract: | A highly sensitive enzyme immunoassay was standardized for aflatoxin B1 determination in poultry feed and its components using Riedel-de-Haen, ELISA Systems. A microtitration plate method was optimized using anti-aflatoxin B1 antibodies and peroxidase – aflatoxin B1 conjugate, based on competitive enzyme immunoassay principle. Standards of concentrations of 5, 10, 20, 50, 100, 500?ng/L aflatoxin B1, prepared in phosphate-buffered saline, were used. Standard curves showed that as the concentration of antigen decreased, absorbance values increased. Fifty percent inhibition was observed at 37?ng/L. Regression analysis showed that log concentration was inversely related to %B/B0, with a highly significant negative correlation (?0.980). The lowest detection limit for aflatoxin B1 was 5?ng/L. Using this standardized ELISA, aflatoxin B1 was detected in most of the commercially available poultry feed samples and their components. The data suggest that this test is suitable for the accurate determination of aflatoxin B1 concentrations in poultry feed and its components. |
