环境水样中双酚A的HPLC检测探讨

研究探讨了各种水化学条件（pH值、离子强度、共存阴离子、共存有机质）对高效液相色谱法（HPLC）检测模拟环境水样中微量双酚A的影响.采用高效液相色谱法检测水样中双酚A的标准曲线线性相关系数高（R2 ＞0.999）,在0.1～5 mg/L的双酚A浓度范围内呈现线性.本研究采用的高效液相色谱法可以在pH 2～11、离子强度0.02 mol/L到2 mol/L NaCl范围内有效地检测双酚A;溶液中的共存阴离子N03-、S04-或共存有机质葡萄糖、单宁酸、没食子酸对检测并没有明显影响;然而当溶液中存在CO32-和p034-时,本研究采用的高效液相色谱法将无法有效检测双酚A,应用时需注意.

Determination of Bisphenol A in Environmental Water Samples with HPLC Method

Bisphenol A in environmental water samples was determined by high performance liquid chromatography （HPLC） method. The effect of different water chemical conditions（ e. g. pH, ionic strength, coexisting anions, coexisting organic matter） on the determination was studied. The calibration curve of determination exhibits a linear range of concentration from 0. 1 to 5 mg/ L, with the correlation coefficient of R2 〉 0. 999. The high performance liquid chromatography method implied in this study can a- chieve good effect for the determination of bisphenol A in pH range of 2 ~ 11 and ionic strength range of 0. 02 ~ 2 mol/L NaC1. The coexisting anions of NO3- and SO42- , and the coexisting organic matter of glucose, tannic acid and gallic acid, have no obvious effect on the determination. However, the existence of COl- and pO34- will make the high performance liquid chromatography method implied in this study fail to determine bisphenol A effectively. This should be taken into consideration in its application.