| 评价饮水DBPs致HepG2细胞损伤的2种试验方法 |
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| 引用本文： | 张绍慧,陈钊,廖静,魏巍,刘爱林,鲁文清.评价饮水DBPs致HepG2细胞损伤的2种试验方法[J].中国环境科学,2010,30(2):275-278. |
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| 作者姓名： | 张绍慧 陈钊 廖静 魏巍 刘爱林 鲁文清 |
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| 作者单位： | 华中科技大学同济医学院,劳动卫生与环境卫生学系,环境与健康教育部重点实验室,湖北,武汉,430030 |
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| 基金项目： | "十一五"国家科技支撑计划 |
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| 摘 要： | 应用结晶紫染色法细胞毒性试验和胞质分裂阻滞法微核试验(CBMNT)评价饮水消毒副产物(DBPs)的HepG2细胞毒性和遗传毒性,探讨该2种试验方法纳入饮水安全性评价体系的可行性.根据遗传毒性和致癌性选用了5种DBPs,分别对HepG2细胞进行染毒,结果表明,5种DBPs的细胞毒性大小排序为3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮(MX)二氯乙腈二溴乙酸二氯乙酸三氯乙酸,其细胞毒性潜力(%C?值)分别为89.74,283.81,1217.02,5066.81,9335.38μmol/L,该5种DBPs在一定浓度下均可致HepG2细胞微核率显著增加,表现出明显的遗传毒性,其遗传损伤能力排序与细胞毒性大小相同.可考虑将这两种实验方法纳入到饮水安全性评价体系中.
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| 关 键 词： | 消毒副产物 细胞毒性试验 微核试验 |
| 收稿时间： | 2009-07-14; |
Application of two assays for damage assessment of damage caused by drinking water disinfection by-products in HepG2 cells |
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| ZHANG Shao-hui,CHEN Zhao,LIAO Jing,WEI Wei,LIU Ai-lin,LU Wen-qing.Application of two assays for damage assessment of damage caused by drinking water disinfection by-products in HepG2 cells[J].China Environmental Science,2010,30(2):275-278. |
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| Authors: | ZHANG Shao-hui CHEN Zhao LIAO Jing WEI Wei LIU Ai-lin LU Wen-qing |
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| Institution: | ZHANG Shao-hui,CHEN Zhao,LIAO Jing,WEI Wei,LIU Ai-lin,LU Wen-qing*(Key Laboratory of Environment , Health,Ministry of Education,Department of Occuptional , Environmental Health,School of Public Health,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China) |
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| Abstract: | Cytotoxicity and genotoxicity of drinking water disinfection by-products(DBPs) in HepG2 cells was to evaluate using crystal violet-colorimetric assay and the cytokinesis-block micronucleus test,and the feasibility of introducing these assays into evaluation system for drinking water safty was explored.HepG2 cells were treated with five DBPs which were chosen based on their genotoxicity and carcinogenicity.The descending rank order in cytotoxicity was 3-chloro-4-(dichloromethyl)-5 hydroxy-2(5 H)-furanone(MX)... |
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| Keywords: | disinfection by-products cytotoxicity assay micronucleus assay |
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