一株Brevibacillus sp.的原生质球的制备及其对Cr(VI)的还原吸附效果分析

利用优势菌生物系统的还原吸附作用去除水环境中的Cr(VI)是一种有前景的处理方法。为了进一步探究优势菌Brevibacillus sp. 还原去除Cr(VI)的机理，利用正交试验得到酶法制备优势菌原生质球的最佳条件，分析原生质球对Cr(VI)的还原吸附效果。结果表明：酶解最佳条件为酶浓度0.1 mg/L，酶解时间30 min，酶解温度30℃。高渗环境下的优势菌原生质球对Cr(VI)和总Cr的去除效率分别为75.90%和63.82%，均高于完整菌株，而相对低渗环境下的原生质球，由于质膜破裂，对Cr(VI)和总Cr的还原吸附去除效率最低。结合Cr(VI)去除率高于总Cr去除率的现象可以推测，可再生原生质球对Cr(VI)的还原吸附过程是一个与其生理活性（透膜机制和代谢活性）相关的过程，且Cr(VI)的还原与吸附是两个联系非常紧密的反应。研究成果为进一步提高Cr(VI)还原去除效率奠定了理论基础。

Preparation of a strain's(Brevibacillus sp.)sphaeroplast and its effect on reduction and adsorption of chromium(VI)

Cr(Ⅵ) removal by the reduction and absorption of the predominated strains-containing bio-sys-tern is one of the most prospective methods. Based on the screening and optimizing of the predominated strain-Brevibacillus sp.,the sphaeroplast was obtained by the enzymic method and the tests on the Cr(Ⅵ) reduction-absorption contribution of the predominated strain's sphaeroplast illuminated that the Cr(Ⅵ) and total Cr remov-al rates of the sphaeroplasts in the liquid with the high osmotic pressure were 75.90% and 63.82%,respective-ly,which were exceeded those of the intact strains,and the removal rates of the sphaeroplasts in the liquid with the relative low osmotic pressure were the least for the break of the plasma membrane. Which may conclude that the process of Cr(Ⅵ) reduction and adsorption by the regenerable sphaeroplasts is relative to the permeation principle and metabolic activity of the plasma membrane,combined with the phenomenon that the removal rates of Cr(Ⅵ) are more than those of the total Cr. The results will be benefit for the further study on the application of Cr(Ⅵ) bio-reduction and removal by the predomated strains.