基于荧光素酶发光检测体系的几种工业污染物综合毒性评价

利用化工污染物能抑制荧光素酶催化发光反应这一特性,对水质中6种化工化合物分别进行毒性测试,建立一种快速检测水质中化工污染物毒性的生物学方法。实验结果表明,对苯二酚、4-氨基酚、苯胺、氯仿、盐酸邻联甲苯胺和硫柳汞的EC50值分别为:5.563、10.225、20.236、38.432、8.827和0.6 mg/L,6种污染物毒性大小依次为:硫柳汞>对苯二酚>盐酸邻联甲苯胺>4-氨基酚>苯胺>氯仿,相关系数>0.95。该方法为进一步研究基于荧光素酶反应体系检测水质中化工污染物综合毒性的生物传感器方法奠定基础。

Determination of synthetic toxicity of chemical contaminants based on inhibition of bioluminescence from luciferase reacting system

A biological method was established for fast determination of the toxicity in water.Six chemical contaminants(hydroquinone,4-aminophenol,aniline,chloroform,o-tolidine dihydrochloride,merthiolate) were used to investigate the toxicity by classic method of equal dose-effect experiment based on the principle that chemical contaminants can inhibit bioluminescence in luciferase reacting system.The results showed that EC50 were 5.563 mg/L for hydroquinone,10.225 mg/L for 4-aminophenol,20.236 mg/L for aniline,38.432 mg/L for chloroform,8,827 mg/L for o-tolidine dihydrochloride and 0.6 mg/L for merthiolate,respectively.Toxicity level order of the chemical contaminants was as follows: merthiolate> hydroquinone> o-tolidine dihydrochlo-ride> 4-aminophenol> aniline>chloroform.The related coefficient was>0.95.This research laid the foundation for the further study of biosensor used to detect the synthesis toxicity of chemical contaminants in water based on bioluminescence from luciferase reacting system.