International standardized procedures for in vivo evaluation of multi-walled carbon nanotube toxicity in water |
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| Authors: | Florence Mouchet Christian Gancet Emmanuel Flahaut Eric Pinelli Jean–Charles Boutonnet Laury Gauthier |
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| Institution: | 1. EcoLab (Laboratoire d'Ecologie Fonctionnelle et Environnement), INP, UPS, Université de Toulouse, Tolosan, France;2. EcoLab, CNRS, Tolosan, France;3. Laboratoire Commun NAUTILE (CNRS–UPS–INPT–ARKEMA France), Laboratoires EcoLab/CIRIMAT/GRL;4. Laboratoire Commun NAUTILE (CNRS–UPS–INPT–ARKEMA France), Laboratoires EcoLab/CIRIMAT/GRL;5. Laboratoire d'Ecotoxicologie, Département Analyse, Groupement de Recherches de Lacq, ARKEMA France, Lacq, France;6. Centre Inter-Universitaire de Recherche et d'Ingénierie des Matériaux – CIRIMAT, UMR UPS INPT CNRS 5085, Toulouse, France;7. Direction Sécurité Environnement Produits, ARKEMA France, Colombes, France |
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| Abstract: | The classical approach in ecotoxicological evaluation of chemical substances consists of conducting standardized bioassays on organism models. In this work, the potential impact of industrial multi-walled carbon nanotubes was investigated by ecotoxicological standardized procedures using aquatic organisms of different trophic levels, namely bacteria, green algae, invertebrates, fish, and amphibians. The results indicated (1) inhibition of growth in amphibians at 50 mg L?1 and higher, and (2) no effects on daphnia and fish up to 100 mg L?1. With the exception of algae (for which Fe deficiency is measured), it seems that the observed toxicity may be due to physiological effects in relation to the ingestion of carbon nanotubes not necessarily related to their intrinsic effects. |
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| Keywords: | Multi-walled carbon nanotubes ecotoxicity standardized bioassays bacteria algae fish amphibians |
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