光谱法研究甲萘威与DNA的相互作用

通过紫外与荧光光谱法研究了甲萘威与DNA的相互作用．结果显示，在甲萘威的作用下，DNA的吸收光谱出现增色与红移现象；DNA能够引起甲萘威单一的静态猝灭；甲萘威与DNA的作用能减弱K4Fe(CN)6)]对甲萘威的荧光猝灭；实验结果支持甲萘威能以嵌插方式与DNA形成加合物，氯化钠及乙醇对加合物的荧光光谱存在不同影响．加合物的形成与药物的生物活性之间存在一定的相互联系。

THE INTERACTION OF CARBARYL WITH CALF THYMUS DNA

Using the technique of UV spectrum and fluorescent quenching respectively,the interaction between carbaryl and ct DNA was studied.The quenching process was proved to be the single static quenching and the quenching constant decreases with temperature increasing.Besides these,the red shift of fluorescent spectrum shows us the information of the intercalation of insecticide into base pairs to produce ct DNA-carbaryl adducts.The UV spectrum showed that ct DNA can also result in the increasing color and red shift.Furthermore,ethanol can produce the change of fluorescent spectrum of ct DNA-carbaryl adducts.At different sodium chloride concentrations,the quenching constant of carbaryl had no significant change,it appeared that there was little chance of electrostatic interaction between ct DNA and carbaryl and it may belong to the mechanism of intercalation.All of above mean the existence of ct DNA-carbaryl adducts,and it is a fact that carbaryl has been considered one potentially mutational substances.