增蓝剂抑制藻类生长效果及对斑马鱼的急性毒性

增蓝剂溶水后降低水下光照度,减弱入射光中370~440nm和530~680nm波段透射光.分析增蓝剂在0.1,1,5mg/L浓度及0.4,0.7,1m水深条件下,对自然水体原生藻类生长抑制效果,并测定其生物毒性.结果表明:在增蓝剂作用下水下光照度衰减明显,衰减系数(k)从0.25m-1增至0.7m-1.水深为0.4,0.7m时,增蓝剂抑藻效果显著(P<0.05),0.1,1,5mg/L浓度对藻类抑制率在第6d后迅速上升,在15d后稳定在65%以上,增蓝剂在0.4m水深抑藻效果较好.当水深至1m时,21d抑藻效果较差.急性毒性试验表明,增蓝剂对斑马鱼96hLC50为442.8 mg/L.

Inhibition effects of true blue on algae growth and its acute toxicity on zebra fish (Brachydanio rerio)

When dissolved in the water, true blue (TB) can lower the underwater intensity of illumination and weaken the incident wave band of 370~440nm and 530~680nm. The inhibiting effect of TB on the algae growth in the concentrations of 0.1mg/L, 1 mg/L and 5mg/L and in water depth of 0.4m, 0.7m and 1m were analyzed, and acute toxic tests of TB on zebra fish (Brachydanio rerio) were also determined. The results showed that the underwater intensity of illumination decayed significantly with attenuation coefficient from 0.25m-1 to 0.7m-1. When in the water depth of 0.4m and 0.7m, algae growth was significantly inhibited (P<0.05) by TB, and the inhibition ratio of 0.1mg/L, 1mg/L and 5mg/L rose rapidly after 6d and were stable over 65% after 15d, the inhibiting effect on the algae in 0.4m groups were better. And the inhibiting effect on algae was poor after 21d when the water depth increased to 1 m under all TB concentrations. The acute toxic tests provided that the 96h LC50 of TB on zebra fish was 442.8mg/L.