Cometabolic degradation of veratryl alcohol and biphenyl by white rot fungus under nitrogen nutrition-rich condition

ＣｏｍｅｔａｂｏｌｉｃｄｅｇｒａｄａｔｉｏｎｏｆｖｅｒａｔｒｙｌａｌｃｏｈｏｌａｎｄｂｉｐｈｅｎｙｌｂｙｗｈｉｔｅｒｏｔｆｕｎｇｕｓｕｎｄｅｒｎｉｔｒｏｇｅｎｎｕｔｒｉｔｉｏｎｒｉｃｈｃｏｎｄｉｔｉｏｎＬｉｎＬｕ，ＤｅｎｇＹａｏｊｉｅ，ＨｕＪｉ...

Cometabolic degradation of veratryl alcohol and biphenyl by white rot fungus under nitrogen nutrition-rich condition

In this paper, changes of enzymes involved in thedegradation of recalcitrant aromatic pollutants from white rotfungus Phlebia radiata I-5-6 and cometabolism of biphenyl andvaratryl alcohol by this fungus under nitrogen nutrition-rich werestudied. Results from the experiment showed that C/N concentrationratio in the culture media played an important role on the activityof LiP. Under the condition of high concentration ratio of C/N orN/C, activity of LiP was higher, but higher activity of MnP onlyfollowed the low concentration of glucose or tatrate ammoniumconcentration in the media, when concentration of glucose ortatrate ammonium was more than 0.01 mol/L, the activity of MnPdropped down quickly. Veratryl alcohol increased the activity of LiP and MnP as well as the amount of .OH radical free underdifferent concentration of nitrogen or carbon source; ascorbicacid, a scavenger of .OH radical free, brought the opposite effectto that of the veratryl alcohol on the LiP and MnP activity. Undernitrogen nutrition-rich condition removal percentage of biphenylwas lower, however, under cometabolic condition of veratryl alcoholand biphenyl, the degradation was enhanced obviously, moreover,intermediated products accumulated in the media during thecometabolic degradation process of biphenyl and veratryl alcoholwas different from these which was found in the process of separatedegradation of biphenyl or veratryl alcohol.