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| 烟草花叶病毒siRNA设计及其植物表达载体构建 | |
| 引用本文： | 马欣荣,谈心,刘华玲,张义正.烟草花叶病毒siRNA设计及其植物表达载体构建[J].应用与环境生物学报,2006,12(2):151-154. |
| 作者姓名： | 马欣荣 谈心 刘华玲 张义正 |
| 作者单位： | 1. 四川大学生命科学学院,成都,610064;中国科学院成都生物研究所,成都,610041 2. 中国科学院成都生物研究所,成都,610041 3. 四川大学生命科学学院,成都,610064 |
| 基金项目： | We are grateful to the Center for Application of Molecular Biology to International Agriculture (CAMBIA),Australia for permitting us to use pCAMBIA2301. |
| 摘 要： | RNA干扰技术已广泛应用于沉默基因表达的研究.本文分析烟草花叶病毒(TMV)基因序列,选择设计编码小分子发卡结构RNA(hpRNA)的cDNA;并根据农杆菌双元载体质粒p2355多克隆位点区限制性酶切位点,两端分别加入XbaI和BamHI酶切位点;分别合成单链DNA复性后插入到p2355上,经PCR、测序验证,表明已成功构建了具有潜在表达烟草花叶病毒siRNA的植物表达载体.图3表1参26 |
| 关 键 词： | RNA干扰(RNAi) 发卡RNA(hpRNA) 小分子干扰RNA(siRNA) 烟草花叶病毒(TMV) 植物表达载体 |
| 收稿时间： | 2005-09-13 |
| 修稿时间： | 2005-11-10 |
Design of siRNAs and Construction of Plant Expression Vector for Interfering with Tobacco Mosaic Virus |
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| MA Xinrong,TAN Xin,LIU Hualing,ZHANG Yizheng.Design of siRNAs and Construction of Plant Expression Vector for Interfering with Tobacco Mosaic Virus[J].Chinese Journal of Applied and Environmental Biology,2006,12(2):151-154. | |
| Authors: | MA Xinrong TAN Xin LIU Hualing ZHANG Yizheng |
| Institution: | 1.College of Life Science, Sichuan University, Chengdu 610064, China;2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China |
| Abstract: | RNA interference (RNAi) is now established as a powerful tool to silence gene expression in a variety of organisms. Depending on the gene sequence of tobacco mosaic virus (TMV), 3 cDNA sequences that could generate short hairpin RNA were chosen and designed. Corresponding to the multiple cloning sites of binary plasmid p2355, XbaI and BamHI were added at the end of each strand, respectively. After being synthesized and annealed, the fragments were inserted into p2355 driven by CaMV35S promotor. Identified by PCR and sequencing, the results showed that 3 vectors with double-stranded RNA-expressing construct were constructed and had potency to produce RNAi against the virus in tobacco. This could be an antiviral strategy in plant. Fig 3, Tab 1, Ref 26 |
| Keywords: | RNA interference (RNAi) hairpin RNA (hpRNA) short interfering RNAs (siRNAs) tobacco mosaic virus (TMV) plant expression vector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |