Comparison of beach bacterial water quality indicator measurement methods |
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| Authors: | Noble Rachel T Weisberg Stephen B Leecaster Molly K McGee Charles D Ritter Kerry Walker Kathy O Vainik Patricia M |
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| Affiliation: | (1) Institute for Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, NC, USA;(2) Southern California Coastal Water Research Project, Westminster, CA, USA;(3) Idaho National Engineering and Environmental Laboratories, Idaho Falls, ID, USA;(4) Orange County Sanitation District, Fountain Valley, CA, USA;(5) Sanitation Districts of Los Angeles County, Carson, CA, USA;(6) City of San Diego Metropolitan Wastewater Department, San Diego, CA, USA |
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| Abstract: | Three methods (membrane filtration, multiple tube fermentation, and chromogenic substrate technology kits manufactured by IDEXX Laboratories, Inc.) are routinely used to measure indicator bacteria for beach water quality. To assess comparability of these methods, quantify within-laboratory variability for each method, and place that variability into context of variability among laboratories using the same method, 22 southern California laboratories participated in a series of intercalibration exercises. Each laboratory processed three to five replicates from thirteen samples, with total coliforms, fecal coliforms or enterococci measured depending on the sample. Results were generally comparable among methods, though membrane filtration appeared to underestimate the other two methods for fecal coliforms, possibly due to clumping. Variability was greatest for the multiple tube fermentation method. For all three methods, within laboratory variability was greater than among laboratories variability. |
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| Keywords: | microbiology intercalibration variability bacterial indicators beach water quality |
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