野生与养殖长吻血液及不同器官同工酶的比较

采用聚丙烯酰胺凝胶电泳（ＰＡＧＥ－Ｅｌｅｃｔｒｏｐｈｏｒｓｉｓ），对野生及养殖长吻Ｕａｉ心脏、肾脏、肌肉、肝脏四个器官及血液的ＬＤＨ（ＥＣ．１．１．１．２７）、ＰＯＤ（ＥＣ．１．１．１．１）、ＥＳＴ（ＥＣ．３．１．１．１）、ＭＤＨ（ＥＣ．１．１．１．３７）同工酶所获电泳图谱进行对比分析，发现以上各酶在不同组织器官中均表现出组织特异性；野生Ｕａｉ和养殖Ｕａｉ四种同工酶也存在差异；ＬＤＨ同工酶电泳带分

COMPARATIVE STUDY ON LSOENZYMES IN CLEIOCASSIS LONG IROSTRIS

The isoenzymes involved in LDH，POD，EST and MDH obtained from heart， kidney， muscle， liver and blood were compared by PAGE-Electrophores is between the wild and raised animals of Cleiocassis longivostris. Tissue s pecificity was found for each isoenzyme from different tissue organs. There were some differences in the four isoenzymes between the wild and raised; LDH isoenz ymes electropherogram was divided into three regions, which were coded by three genes. Five basic bands exsisting in L2 regions a4 and b4 were shown with ance stry characteristic. EST isoenzymes electropherogram was in ploymorphism,which w as very complicated. The wild was different from the raised in activity of POD a nd MDH, showing the state of aerobic metabolism. It is found that the four i soenzymes are regarded as the important reference indexes for identifying ger m plasm res ources, analysing biochemical heredity, classifying systems and studying th e deveopment of metabolism. Fig 4, Ref 13