| 沙雷氏菌发酵蓝藻生产蛋白酶 |
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| 引用本文： | 李桂英,蔡宇杰,廖祥儒,尹静.沙雷氏菌发酵蓝藻生产蛋白酶[J].环境工程学报,2012,6(3):1043-1046. |
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| 作者姓名： | 李桂英 蔡宇杰 廖祥儒 尹静 |
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| 作者单位： | 江南大学生物工程学院工业微生物国家重点实验室,无锡,214036 |
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| 基金项目： | 国家“863”高技术研究发展计划项目（2010AA101501） |
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| 摘 要： | 以富含蛋白质的蓝藻作有机氮源进行微生物发酵生产蛋白酶,为蓝藻的资源化利用提供一条新途径。采用单因素实验,研究了影响沙雷氏菌发酵蓝藻生产蛋白酶的培养基主要成分。结果发现,在实验范围内,沙雷氏菌发酵蓝藻生产蛋白酶的最佳碳源是蔗糖,最佳速效氮源是尿素,最佳金属离子(盐)是ZnSO4,最佳产酶促进剂是吐温80。在单因素实验中,发酵上清液的蛋白酶活最高可达到941 U/mL,而且发酵周期短,发酵培养18 h即可达到最高酶(活)值。
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| 关 键 词： | 蛋白酶 蓝藻 沙雷氏菌 单因素实验 |
Protease production by Serratia sp. SYBC H with blue algae |
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| Li Guiying,Cai Yujie,Liao Xiangru and Yin Jing.Protease production by Serratia sp. SYBC H with blue algae[J].Techniques and Equipment for Environmental Pollution Control,2012,6(3):1043-1046. |
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| Authors: | Li Guiying Cai Yujie Liao Xiangru and Yin Jing |
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| Institution: | Li Guiying Cai Yujie Liao Xiangru Yin Jing(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122,China) |
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| Abstract: | The microbial fermentation of protein-rich blue algae for proteases production provides a new way to utilize the blue algae resource.The main factors of the fermentation medium affecting proteases production were studied by Serratia sp.SYBC H via the single factor experiment.The results showed that within the experimental ranges,the optimal carbon source,inorganic nitrogen source,salt and stimulant for Serratia sp.SYBC H proteases production were sucrose,urea,ZnSO4 and Tween 80,respectively.The highest protease activity reached 941 U/mL in the supernatant.The fermentation time is short.Only 18 h is required to reach the maximum protease activity in the supernatant. |
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| Keywords: | protease blue algae Serratia sp SYBC H single factor experiment |
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